Acvrl1 (NM_001277258) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Acvrlk1; AI115505; AI427544; Alk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228058 representing NM_001277258 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTTGGGGAGCTTCAGAAGGGGCCTTTTGATGCTGTCGGTGGCCTTTGGCCTAACCAGGGGAGACT TGGCGAAGCCTTCCAAGCTGGTGAACTGCACTTGTGAGAGCCCACACTGCAAGAGACCATTCTGCCAGGG GTCATGGTGCACAGTGGTGCTGGTTCGGGAGCAGGGCAGGCACCCCCAGGTCTATCGGGGCTGCGGGAGC CTGAACCAGGAGCTCTGCTTGGGACGTCCCACAGAGTTTCTGAACCATCACTGCTGCTATAGATCCTTCT GCAACCACAACGTGTCTCTGATGCTGGAGGCCACCCAAACTCCTTCGGAGGAGCCAGAAGTTGATGCCCA TCTGCCTCTGATCCTGGGTCCTGTGCTGGCCTTGCCGGTCCTGGTGGCCCTGGGTGCTCTAGGCTTGTGG CGTGTCCGGCGGAGGCAGGAGAAGCAGCGGGATTTGCACAGTGACCTGGGCGAGTCCAGTCTCATCCTGA AGGCATCTGAACAGGCAGACAGCATGTTGGGGGACTTCCTGGACAGCGACTGTACTACGGGCAGCGGCTC GGGGCTCCCCTTCTTGGTGCAGAGGACGGTAGCTCGGCAGGTTGCGCTGGTAGAGTGTGTGGGAAAGGGC CGATATGGTGAGGTGTGGCGCGGTTCGTGGCATGGCGAGAGTGTGGCGGTCAAGATTTTCTCCTCACGAG ATGAGCAGTCCTGGTTCCGGGAGACGGAGATCTACAACACAGTTCTGCTTAGACACGACAACATCCTAGG CTTCATCGCCTCCGACATGACTTCGCGGAACTCGAGCACGCAGCTGTGGCTCATCACCCACTACCATGAA CACGGCTCCCTCTATGACTTTCTGCAGAGGCAGACGCTGGAGCCCCAGTTGGCCCTGAGGCTAGCTGTGT CCGCGGCCTGCGGCCTGGCGCACCTACATGTGGAGATCTTTGGCACTCAAGGCAAACCAGCCATTGCCCA TCGTGACCTCAAGAGTCGCAATGTGCTGGTCAAGAGTAACTTGCAGTGTTGCATTGCAGACCTGGGACTG GCTGTGATGCACTCACAAAGCAGCGATTACCTGGATATCGGCAACAACCCCCGAGTGGGTACCAAAAGAT ACATGGCACCCGAGGTGCTGGATGAGCACATCCGCACAGACTGCTTTGAGTCGTACAAGTGGACAGACAT CTGGGCCTTTGGCCTAGTGCTATGGGAGATCGCCCGGCGGACCATCATCAATGGCATTGTGGAGGATTAC AGGCCACCTTTCTATGACATGGTACCCAATGACCCCAGTTTTGAGGACATGAAAAAGGTGGTGTGCGTTG ACCAGCAGACACCCACCATCCCTAACCGGCTGGCTGCAGATCCGGTCCTCTCCGGGCTGGCCCAGATGAT GAGAGAGTGCTGGTACCCCAACCCCTCTGCTCGCCTCACCGCACTGCGCATAAAGAAGACATTGCAGAAG CTCAGTCACAATCCAGAGAAGCCCAAAGTGATTCACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277258
Insert Size	1509 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277258.1, NP_001264187.1
RefSeq Size	3571 bp
RefSeq ORF	1509 bp
Locus ID	11482
UniProt ID	Q61288
Gene Summary	Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 4 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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