Shmt2 (NM_001252316) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2700043D08Rik; AA408223; AA986903
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228056 representing NM_001252316 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTATCCTTCTCTTTGCTTCGGACCACTCGGAGATGTGGTCAGCTGGTCTGCATGGCTGCCCGGGCCC AGCACAGCAAGGTGGCCCAGACGCAGGCTGGGGAAGCAGCTGGAGGTTGGACGGGCCAGGAGAGTTTATC AGACAGTGACCCTGAGATGTGGGAGCTTCTGCAGAGGGAGAAGGACAGACAGTGTCGCGGCCTGGAGCTC ATCGCCTCAGAGAACTTCTGCAGCCGAGCTGCGCTGGAGGCCCTGGGGTCCTGTCTCAACAACAAGTACT CGGAGGGTTACCCTGGCAAGAGATACTACGGAGGAGCGGAAGTGGTGGACGAGATCGAGCTGCTCTGCCA GCGCCGGGCCTTGGAAGCCTTTGACCTGGATCCGGCACAGTGGGGAGTCAATGTGCAGCCATACTCAGGG TCCCCAGCCAATCTGGCTGCCTATACGGCCCTTCTGCAGCCTCATGATCGAATCATGGGGTTGGACCTGC CCGATGGGGGCCATCTCACCCATGGCTACATGTCTGATGTCAAGCGGATCTCCGCCACATCCATTTTCTT CGAGTCTATGCCCTATAAGCTCAATCCCCAAACTGGCCTCATCGACTACGACCAGCTGGCGCTGACCGCT CGGCTTTTCCGACCGCGGCTCATCATAGCTGGCACGAGTGCCTATGCCCGCCTCATTGACTATGCACGCA TGAGAGAGGTCTGTGATGAGGTCAGGGCACACCTGCTGGCAGACATGGCCCACATCAGTGGCCTGGTGGC TGCCAAGGTGATCCCCTCCCCTTTCAAGTACGCGGATGTTGTTACCACCACCACTCACAAGACACTGCGA GGGGCCAGGTCAGGGCTCATCTTCTACCGGAAGGGAGTACGAACCGTAGACCCCAAGACTGGCAAAGAGA TCCCTTATACCTTTGAGGACCGAATCAACTTCGCTGTGTTCCCATCCCTACAGGGTGGCCCCCACAACCA CGCCATTGCTGCAGTAGCCGTGGCTCTCAAGCAGGCCTGCACCCCTATGTTCCGCGAGTACTCCTTACAA GTGCTGAGGAACGCCCAGGCCATGGCTGATGCCCTGCTCAAGCGAGGATACTCGCTGGTGTCTGGTGGCA CTGACACCCACCTGGTGCTGGTGGACCTGCGGCCCAAGGGCCTGGATGGAGCCCGAGCCGAACGCGTGTT GGAACTCGTCTCCATCACAGCCAACAAGAACACCTGTCCTGGAGACCGGAGCGCCATTACTCCGGGGGGC TTGAGGCTTGGGGCCCCCGCGTTGACCTCTCGCCAGTTCCGTGAGGACGACTTCCGTAGAGTCGTCGATT TTATCGATGAGGGAGTCAACATTGGCTTGGAGGTGAAGCGCAAGACTGCCAAGCTCCAGGATTTCAAATC CTTCCTGCTCAAAGACCCAGAGACAAGCCAGCGTTTGGCCAACCTCCGGCAACAGGTGGAACAGTTTGCC AGGGGCTTCCCCATGCCTGGATTTGATGAACGTTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001252316
Insert Size	1506 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252316.1, NP_001239245.1
RefSeq Size	2296 bp
RefSeq ORF	1506 bp
Locus ID	108037
UniProt ID	Q9CZN7
Gene Summary	Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (By similarity). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (By similarity). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (By similarity). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29452640). Associates with mitochondrial DNA (By similarity). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses a different splice site in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus when it is compared to isoform 1.

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