Dach2 (NM_001289732) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9430028N04Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228052 representing NM_001289732 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAAGGAAACAAGCTGTTAACAGTTCAAGGCCTGGCAGGCCCCCTAAGCGTTCTTTGGGAGTATTGC AAGATAATGCCCGACTTCTGCCCCATGCAGTCCCAGGCCTCTTATCACCAGGACTGATCACTCCAACAGG TATCACAGCTGCAGCGATGGCTGAGGCCATGAAGCTCCAGAAGATGAAGCTTATGGCAATGAATACTCTT CAGGGTAATGGAAGCCAAAATGGGACTGAGTCAGAGCCAGATGACCTTAATTCTACGACAGGTGGAAGTG AATCCTCCTGGGATAAAGATAAGATACAATCTCCACTTGCTGCTTCTGGACCTCAACATGGAATTGCTCA TGCAGCACTTGCTGGTCAGCCAGGCCTTGGAGGTGCTCCGACCCTTAATCCACTTCAGCAGAATCACCTG CTAAGCAATCGTCTGGATCTCCCATTTATGATGATGCCTCATCCCCTACTTCCAGTCAGCTTACCTCCTG CATCAGTTGCCATGGCAATGAATCAGATGAATCATCTCAATACTATTGCCAACATGGCTGCTGCAGCCCA GATTCACAGTCCACTCTCCAGAGCTGGTGCCTCTGTTATAAAGGAACGGATCCCAGAGAGTCCTTCTCCT GCTCCCTCTCTGGAAGAGAGTCATCGTCCTGGGAGCCAGACCTCCTCCCACCCAAGCAGCAGTGTGTCCA GCTCTCCCTCGCAGATGGATCATCATTCAGAGAGAATGGTTATGATGCCCAACAATCGAGAAGAGCTTAT TGTTGACCAAGATAATGGACAAAGCATAAAAAAATTCCAGAGGGATAATAAAGATATCCAATTGTCCCAG CACCATTTGTTGAACACTTTTCTTCACTGGATTGAGTTGGCACCATTATCAAAAAAATCACTTCACCATC AAAAAGAAGTACCAGCTCAAATCCCAGTCATGAAGTCACCCTTGGATAAGATCCAGTTGGCTCCTGGACA GGCATTGCATCCTGGATTCCCTGGACCATTCATTTTTGCAGATAGTCTATCTTCTGTGGAGACTCTGTTG ACCAACATTCAGGTCAACAATATTTCTATAAACAAAATAAATGGTCTACTGAAAGTGGCTTTGGATAATG CTCGTATCCAGGAGAAGCAGATTCAGCAGGAAAAGAAGGAACTGCGAATAGAGCTCTTCAGAGAAAGAGA AATTAGAGAAAACCTGGAGCGACAACTTGCAGTTGAGCTTCAAAGCAGAAGTACAATGCAAAAGCGTCTG AAAAAAGAGAAAAAGGCTAAGAGAAAACTTCAGGAAGCCTTGGAATTTGAATCAAAGCGCCGCGAGCAAG TGGAGCAAGCACTTAAACAAGCTACATCTGGTGACAGTGGACTGAGAATGTTAAAAGATTCTGGAATTCC AGATATTGAAATAGAAAACAGTGGAACGCCGCATGACAGTGCTGCTATGCAAGGAGGTAACTATTACTGT TTAGCAATGGCACAGCAGTTGTGTTCAGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289732
Insert Size	1503 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289732.1, NP_001276661.1
RefSeq Size	2894 bp
RefSeq ORF	1503 bp
Locus ID	93837
UniProt ID	Q925Q8
Gene Summary	Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at an alternate start codon, compared to variant 1. The encoded protein (isoform 3) is shorter and has a distinct N- and C-terminus, compared to isoform 1.

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