Osbp2 (NM_001302631) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700095P05Rik; C630001G20Rik; Gm244; ORP-4; OSBPL1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228044 representing NM_001302631 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCCTGGAGAAGGCCAGAGGCAGCCAAGAAGACCCTGGGAGTTGGGGTGGAAGCCAGGAAGCTTCT TGACTTCCAAAGGAGAGAACAGTGAGGAAGATGAAGATACTGAGTACTTTGACGCCATGGAAGACTCTAC ATCTTTCATCACCGTAGTCACTGAGGCCAAGGAAGACAGAAAGCCTGAGAGTGGACCTGGGACCACCACT GTGGACTGGACCTCAGCAGATAATGTATTAGATGGTGCCTCATTCATGCCCAAGAATTCATGCAAAGTTA AGAGGCGAGTCCGCATCCCTGACAAACCCAACTATAGCCTTAACCTCTGGAGCATCATGAAGAATTGTAT TGGCCGAGAGCTTTCCCGGATCCCCATGCCGGTGAACTTCAATGAGCCCCTGTCCATGCTCCAGCGACTT ACAGAGGACCTGGAGTACCACCACCTGCTGGACAAGGCGGTGAACTGCACCAGCTCGGTGGAGCAGATGT GCCTGGTAGCCGCCTTTTCTGTGTCCTCCTACTCCACCACGGTGCACCGCATCGCCAAGCCCTTCAACCC TATGCTCGGGGAGACCTTCGAGCTGGACCGTATGGAGGACATGGGCCTGCGTTCCCTCTGTGAGCAGGTG AGCCACCACCCCCCGTCTGCTGCCCACCACGTGTTCTCTAAGCATGGCTGGAGCCTCTGGCAAGAAATCA CCATCGCCAGCAAGTTCCGAGGGAAATACATCTCTATCATGCCACTCGGTGCCATCCACCTAGAATTCCA GGCCAGTGGCAATCACTACGTGTGGAGGAAGAGCACCTCCACCGTGCACAACATCATCGTGGGCAAGCTC TGGATTGACCAGTCAGGGGACATTGAGATTGTGAACCACAAGACCAAGGACCGGTGCCAGCTGAAGTTCG TACCCTACAGCTACTTCTCCAAAGAGGCAGCCCGAAAGGTGACTGGAGTGGTGAGTGACAGCCAGGGCAA GGCCCACTACGTGCTGTCAGGTTCGTGGGATGACCAGATGGAATGTTCTAAGATTGTGCACAGCAGCCCC AGCTCTGATGGGAGACAGAAAACCGTGTACCAGACACTGCCCGCCAAACTGCTCTGGAGGAAATACCCAC TGCCGGAGAATGCGGAGAACATGTACTATTTCTCCGAGCTAGCCCTGACCCTCAACGAGCAGGAGGACGG CGTGGCGCCCACCGACAGTCGCCTGCGGCCAGACCAGCGGCTTATGGAGAGGGGACGCTGGGACGAGGCC AACACCGAGAAGCAACGGCTGGAGGAGAAGCAGCGCCTGTCCAGGCGACGGCGGCTGGAGTCATGCACGG CAGGCTGCGGTGGGGAGGAAGCAGAGAAGGAGTCAGATGGCTATGTGCCGCTCTGGTTCGAGAAGAGGCT GGACCCGCTGACTGGGGAGATGGCCTGCATGTACAAGGGCGGCTACTGGGAGGCCAAGGAGAAGAAGGAC TGGCACATGTGCCCCAACATCTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302631
Insert Size	1497 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302631.1, NP_001289560.1
RefSeq Size	2916 bp
RefSeq ORF	1497 bp
Locus ID	74309
Gene Summary	The protein encoded by this gene belongs to the oxysterol-binding protein-related family of proteins, which are defined by a C-terminal sterol domain with a highly conserved EQVSHHPP motif. Oxysterols are oxygenated derivatives of cholesterol that are involved in mechanisms that include apoptosis, cholesterol homeostasis, lipid trafficking and cell differentiation. This protein is selectively expressed at high levels in the brain and testis. Within the testis, the mRNA is localized to postmeiotic germ cells, including spermatids and spermatozoa, but is not detectable in somatic cells. Mice homozygous mutant for a targeted deletion in this gene do not exhibit overt developmental phenotypes but are male sterile. Females display normal fertility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.

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