Btbd35f21 (NM_001270669) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gm21951; Mgclh
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228042 representing NM_001270669 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTTTTAGTCAGCAGGGTCTTGAGATGCAGGGATTCCAGTCTGTTAGAGCCACAGCCAGAAGCCA TAGCCGGAGCCAGCTACATTCCTGGCAGTCGCAAGCGAAAAAGAAACAGTTTGGAGGAGTTGGCAACAAG TTCTAATGTTCATGGACCTCAAAACCAGGGAATGTATCCACATCAAGTTCTCAACTACATCTACTGGAAA AGGGTTAAGATCTCATCTAATGATGCTTATCAAAACTTATTTTTGGACGGGCATGATAGCGACATTAAAA TCCGTGCTCTGGGAAGAACATGGTGTTTACACAAAGTATTTTTATGTCAGTCAGGCTACTTTGCTAACAT TCTCAAAGGTACTTGGAGAGAATCACACCATGGTGTTATAAATCTGATCATTAAGAATGAGGATATTGAT ACCCGATCTCTGCATTTTGTGTTTGGTGCTCTGTACACGGATGCGGATTTGTCAATAACACCTCTGGAAG TTCCTCAAGTTTTGGCAGCAGCATGCCTGCTTCGAGTGGATCGAGTAATTCAGCAGTGTGAAGGAATCAT GAAAGAAACTATCAACAGGAACACTGTGTGCTCCTATTATTTGGCAGCAGAAACCTATAGATTAAAAGCT GTAAAGACGAGATGCTTTGAATGGCTTCTTTGCAATTTGATGGTACATCCAAGTGTGGCACTTTACAAGG AAGTAGATATGAAGTTGATGTATCTTCTAGCACTGTCTTCTGACTTACTAGTCATGCAAAAGGAGATTGA TGTATATACCACACTAAAAATATGGATGATCCTTTATCTTAATCCATGCTGGAACGGAACCATGAAACAG CTTTTACAACACGCAAACAACTGGCTTTCCACCCACATGGCATATATTGATAACATCAGTTTTCTTGAAA GTGAAGAAGGACTAATATTTCAACCAGTGTTTAAAAAGCTGAGATTTCAGCACATCATCTGTGACTTGAC TTCCACAACTATTCTTGAACAAGATCGACTAATACCTATGGCATGGTTGTCACCCATTTACAAACAACAG TGGTTGACTTTGCTGCGAACACAAGAATATGGGGTAATTGGACCACAAGTTATCAATGAACAAGAACTTG AAGAATGCACCATGAGGTGTGGTACAATGATCCCCAAAGATGGAAGATATACTTGGAAGTGGTCAGTTGG ACGACTTGGCTTTCCTTTACGTGTGACCTTTACCAGGCAGTGTGTAATTTTAAGGCAACGGTGTCAGAGG TGTGATGGTTCTGCTTGCCACAACCATATCCGAAATGTCATATTCAGAATAACTTTGGTGTGTTTTGATT CCAACAAAAGAGTAACTTTCAGAAAGACAACAGGTTATAAAATCCTCACCTTTGAATATAACGAGGAGCA AATTGTAATGAAATTGGATAGTGATGTTCTAACCTTCCCTATGTGTATATTCTGCAATTTCCTTTTTGTA AACCTAGGAAATGCAGAAAACAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270669
Insert Size	1497 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270669.1, NP_001257598.1
RefSeq Size	1921 bp
RefSeq ORF	1497 bp
Locus ID	100862329
UniProt ID	Q99N64
Gene Summary	Possible function in spermatogenesis. Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.[UniProtKB/Swiss-Prot Function]

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