Angpt1 (NM_001286062) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110046O21Rik; an; Ang; Ang-1; Ang1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228032 representing NM_001286062 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGTTTTCCTTTCCTTTGCATTCTTCGCTGCCATTCTGACTCACATAGGGTGCAGCAACCAGCGCC GAAATCCAGAAAACGGAGGGAGAAGATATAACCGGATTCAACATGGGCAATGTGCCTACACTTTCATTCT TCCAGAACACGACGGGAACTGCCGTGAGAGTGCGACAGAGCAGTACAACACCAACGCTCTGCAAAGGGAT GCTCCACACGTGGAGCCGGATTTCTCTTCCCAGAAACTTCAGCATCTGGAGCATGTGATGGAAAATTATA CTCAGTGGCTGCAAAAACTTGAGAATTACATTGTGGAAAATATGAAGTCGGAGATGGCCCAGATACAACA GAATGCTGTTCAAAACCACACGGCCACCATGCTTGAGATAGGAACCAGTCTCTTATCTCAGACTGCAGAG CAGACCCGAAAGCTGACAGATGTTGAGACCCAGGTACTAAATCAAACATCCCGACTTGAAATACAACTGC TAGAGAATTCATTATCAACATACAAGCTAGAGAAGCAACTTCTCCAACAGACAAATGAAATTCTGAAGAT TCACGAAAAAAACAGTTTACTAGAGCACAAAATCTTAGAAATGGAGGGAAAACACAAAGAAGAATTGGAC ACCTTGAAGGAGGAGAAAGAAAACCTTCAAGGCTTGGTTTCTCGTCAGACATTCATCATCCAGGAGTTGG AGAAGCAACTTAGTAGAGCTACCAACAACAACAGCATCCTGCAGAAGCAACAACTGGAGCTCATGGACAC AGTTCATAACCTTATCAGCCTTTGCACTAAAGAAGTTTTGCTAAAGGGAGGAAAAAGAGAAGAAGAGAAA CCATTTCGAGACTGTGCAGATGTATATCAAGCTGGTTTTAATAAAAGTGGAATCTACACTATTTATTTTA ATAATATGCCAGAACCCAAAAAGGTATTTTGCAATATGGATGTGAATGGGGGAGGTTGGACAGTAATACA ACACCGGGAAGATGGAAGCCTGGATTTCCAGAGGGGCTGGAAGGAGTATAAAATGGGTTTTGGGAATCCC TCTGGTGAATATTGGCTTGGGAACGAGTTCATTTTTGCAATAACCAGTCAGAGGCAGTACATGCTGAGGA TTGAGCTGATGGACTGGGAAGGGAACCGAGCCTACTCACAGTACGACAGATTCCACATAGGAAATGAAAA GCAGAACTATAGGTTATATTTAAAAGGTCACACAGGGACAGCAGGCAAACAGAGCAGCTTGATCTTACAC GGTGCCGATTTCAGCACGAAGGATGCTGATAACGACAACTGTATGTGCAAATGCGCTCTCATGCTAACAG GAGGTTGGTGGTTCGATGCCTGTGGCCCTTCCAATCTAAATGGAATGTTCTACACTGCGGGACAAAATCA TGGAAAACTGAATGGGATAAAGTGGCACTACTTCAAAGGGCCCAGTTACTCCTTACGTTCCACCACCATG ATGATCCGGCCCTTGGACTTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286062
Insert Size	1494 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286062.1, NP_001272991.1
RefSeq Size	4313 bp
RefSeq ORF	1494 bp
Locus ID	11600
Gene Summary	This gene encodes a secreted glycoprotein that belongs to the angiopoietin family of vascular growth factors. The encoded protein is a ligand in the vascular tyrosine kinase signaling pathway and regulates the formation and stabilization of blood vessels. This protein also functions in striated muscles by promoting proliferation, migration and differentiation of skeletal myoblasts and plays an essential role in the vascular response to tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region compared to variant 1. This encoded protein (isoform 2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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