Sgk3 (NM_133220) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2510015P22Rik; A330005P07Rik; Cisk; fy; fz
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228022 representing NM_133220 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGGGATTGTATCATGGACTACAAGGAGAGCTGCCCAAGTGTAAGCATTCCCAGCTCTGACGAAC ACAGAGAGAAAAAGAAGAGGTTCACGGTTTATAAAGTTCTGGTCTCTGTGGGCAGAAGCGAGTGGTTTGT CTTCAGGAGATACGCAGAGTTTGACAAACTTTACAATTCTTTAAAGAAGCAGTTTCCTGCTATGGCTCTG AAGATTCCTGCCAAGAGAATATTTGGTGATAATTTTGATCCAGATTTTATTAAACAAAGAAGAGCAGGAT TGAATGAGTTCATTCAGAACTTGGTCAGATATCCAGAGCTTTACAACCATCCAGATGTCCGAGCATTCCT TCAAATGGACAGCCCAAGACATCAGTCAGATCCATCTGAAGATGAGGATGAAAGAAGTACTTCGAAGCCA CATTCTACCTCACGGAACATCAACCTGGGACCAACTGGAAATCCTCATGCTAAACCAACTGACTTCGATT TTTTAAAAGTTATTGGAAAGGGCAGCTTTGGCAAGGTTCTTCTTGCAAAACGGAAACTGGATGGAAAATT TTATGCTGTCAAAGTGTTACAGAAAAAAATAGTTCTCAACAGAAAAGAGCAAAAACATATTATGGCTGAA CGCAATGTGCTCTTGAAAAATGTGAAGCACCCATTTTTGGTTGGATTGCACTATTCTTTCCAAACAACTG AAAAGCTTTATTTTGTTCTGGATTTTGTTAATGGAGGGGAGCTCTTCTTTCACCTCCAAAGGGAAAGGTC TTTTCCTGAACCCAGAGCGAGGTTTTATGCCGCGGAGATCGCCAGTGCCTTGGGCTACCTGCACTCCATC AAAATAGTGTACAGAGACTTGAAGCCAGAAAATATTCTTTTGGATTCAATGGGACATGTTGTCTTAACGG ATTTTGGACTTTGCAAAGAAGGAATCGCTATTTCTGATACCACCACAACTTTTTGTGGTACACCAGAGTA CCTTGCACCTGAAGTAATCAGAAAACAGCCCTATGACAACACTGTGGACTGGTGGTGCCTGGGCGCTGTT CTGTATGAGATGCTGTACGGGCTGCCTCCTTTTTACTGCCGAGATGTTGCTGAAATGTATGACAATATTC TTCACAAGCCCTTAAACTTGAGACCAGGAGTGAGTCTCACCGCCTGGTCCATTCTGGAAGAACTTCTAGA AAAAAACAGACAAAATCGACTTGGTGCCAAAGAAGACTTTCTTGAAATCCAGAATCATCCTTTTTTTGAG TCACTCAGCTGGACTGACCTCGTACAAAAAAAGATTCCACCTCCATTTAACCCTAATGTGGCTGGACCAG ATGATATCAGAAACTTTGATGCCGTCTTCACTGAAGAAACGGTTCCCTATTCAGTGTGTGTGTCTTCTGA CTATTCCATCGTGAATGCCAGTGTTCTGGAGGCAGATGATGCATTTGTTGGTTTTTCTTACGCCCCTCCT TCGGAAGACTTATTTTTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_133220
Insert Size	1491 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_133220.2, NP_573483.1
RefSeq Size	5517 bp
RefSeq ORF	1491 bp
Locus ID	170755
UniProt ID	Q9ERE3
Gene Summary	Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.

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