Agbl4 (NM_001284190) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930578N11Rik; 4931433A01Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228013 representing NM_001284190 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCGGAGCCAGACAGCGCCAGAGGCAGGCAATGATACAGGAAATGAGGATGCCATTGGAGGGA ATGTGAACAAATACATAGTGCTTCCAAATGGATACTCTGGACAGCCCAAGAAAGGACATCTTACCTTTGA TGCTTGCTTTGAAAGTGGTAACCTCGGCCGGGTTGAGCAAGTCTCTGATTTTGAGTATGATCTGTTCATT AGGCCGGACACCTGTAATCCACGCTTCCGAGTCTGGTTCAACTTTACTGTTGAAAATGTGAAAGAATTGC AGAGGGTAATTTTCAACATTGTTAACTTCAGTAAAACCAAGAGTCTTTACCGAGATGGGATGGCACCAAT GGTGAAATCTACCAGCAGACCAAAATGGCAAAGACTACCACCAAAAAACGTTTATTACTATCGCTGCCCA GACCACAGGAAGAACTATGTGATGTCCTTTGCATTCTGTTTTGACCGAGAAGATGATATCTACCAGTTTG CTTACTGCTACCCTTATACATATACTCGCTTCCAGCATTACCTCGACAGCTTACAAAAGAAAAACATGGA TTATTTCTTCCGGGAACAGCTTGGACAGAGTGTGCAACAGCGGCAGCTTGACCTCCTGACGATAACCAGC CCGGAGAATCTCCGTGAAGGGTCAGAAAAGAAGGTGATATTCATCACAGGGCGAGTCCACCCAGGGGAAA CGCCATCTTCATTTGTGTGCCAAGGAATCATCGACTTCCTTGTAAGTCAGCATCCAATTGCCCGTGTCCT ACGAGAACATTTAGTCTTCAAGATTGCTCCAATGCTCAACCCTGATGGAGTTTACCTGGGCAACTACAGG TGTTCCCTGATGGGGTTTGACCTGAATCGTCACTGGCTGGATCCCTCTCCATGGGCCCATCCCACCCTGC ATGGAGTGAAACAGCTTATTATCAAGATGTACAATGACCCAAAAACAAGCCTGGAGTTCTATATTGACAT CCACGCTCACTCCACCATGATGAATGGCTTCATGTACGGCAATATCTTTGAGGATGAGGAACGGTTCCAA AGGCAGTCCATTTTTCCAAAACTCCTTTGCCAGAATGCCGAGGACTTCTCCTATACTAGCACATCCTTCA ACAGGGATGCTGTGAAAGCAGGAACTGGCCGGCGCTTCCTGGGCGGGCTTCTGGACCACTCATCATACTG CTATACCCTAGAGGTTTCCTTCTACAGCTACATCATTGGGGGTACTACAGCTGCAGTGCCCTACACTGAA GAAGCCTATATGAAACTGGGACGGAACGTGGCAAGAACATTTCTAGATTATTACCGGCTGAACTCCCTGG TTGAGAAGATAGCAGTCCCCATGCCAAGACTTCGGAAAGAAAAAGCCCCTCCTTGCAAGCACCCACCCCC AAGGGGCTCAACCAGCAACGTCTCCATTGGCAAAGGGGACAAGAAGAACTCACTGAACCACAAAGACCCA TCAACCCCCTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001284190
Insert Size	1485 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284190.1, NP_001271119.1
RefSeq Size	1659 bp
RefSeq ORF	1485 bp
Locus ID	78933
Gene Summary	Metallocarboxypeptidase that mediates deglutamylation of target proteins (PubMed:17244818, PubMed:21074048, PubMed:25103237, PubMed:26829768). Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins (PubMed:17244818). Also removes polyglutamates from the carboxy-terminus of target proteins such as MYLK (PubMed:21074048). Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS (PubMed:26829768). Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5 (PubMed:25103237).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate 3' exon structure compared to variant 1. It encodes isoform 3, which is shorter and has a distinct C-terminus, compared to isoform 1.

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