Celf3 (NM_001289613) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930415M08Rik; BRUNOL1; CAGH4; ERDA4; Tnrc4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228012 representing NM_001289613 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAGCCAGATGCCATCAAGCTGTTTGTGGGGCAGATCCCGAGGCATCTGGAGGAAAAGGACCTGA AGCCCATCTTCGAGCAGTTTGGTCGGATCTTCGAGCTGACTGTCATCAAGGACAAGTACACCGGGCTGCA CAAGGGATGTGCTTTCCTGACGTACTGTGCTCGCGATTCAGCCCTGAAGGCCCAGAGTGCCCTGCACGAA CAGAAGACTCTCCCAGGGATGAACAGGCCGATCCAGGTCAAGCCAGCCGACAGCGAGAGTCGAGGAGAAG ACCGGAAGCTCTTTGTGGGCATGCTAGGAAAGCAGCAGACAGATGAGGATGTCCGGAAGATGTTTGAACC ATTTGGGACTATAGACGAGTGCACTGTGCTCCGGGGGCCAGACGGTACCAGCAAAGGCTGTGCCTTTGTG AAGTTCCAGACTCACGCTGAGGCCCAGGCAGCCATCAACACCCTTCACAGCAGCCGGACCCTACCGGGTG CCTCATCCAGCCTGGTGGTAAAGTTTGCTGACACGGAGAAGGAGCGAGGTCTCCGTCGAATGCAGCAGGT GGCTACCCAGCTGGGCATGTTCAGCCCGATCGCCCTCCAGTTTGGAGCCTACAGCGCCTACACCCAGGCC CTGATGCAGCAGCAGGCGGCCCTGGTAGCAGCTCACAGTGCCTACCTCAGCCCTATGGCCACCATGGCTG CCGTGCAGATGCAGCACATGGCTGCCATCAGTGCCAATGGCCTCATCGCCACCCCCATCACTCCATCCTC AGGAACCAGCACCCCTCCTGCCATTGCTGCCACGCCCGTCTCTGCCATCCCTGCTGCCTTGGGCGTCAAC GGCTACAGCCCGGTGCCCACCCAGCCTACAGGGCAGCCTGCCCCGGATGCTCTGTATCCCAACGGGGTTC ACCCTTACCCAGATGAGGCTCTGTCTGCTGAGAGAAGTGCTGGCGGGGTTCCCATAATGTCCCAGGCCCA CTCATGGCTTGTGATGCTCTCTGCAGCCCAGAGCCCGGCAGCCCCCGTGGACCCTCTCCAGCAGGCCTAT GCAGGAATGCAGCACTACACAGCAGCGTACCCCGCAGCCTACAGCCTGGTTGCACCTGCGTTCCCGCAGC CTCCAGCCCTGGTTGCCCAGCAGCCCCCACCACCACCTCAGCAACAGCAGCAGCAGCAGCAGCAGCAACA GCAACAGCAGCAGCAACGGGAAGGCCCTGATGGCTGCAACATCTTCATCTACCACCTGCCCCAGGAGTTC ACAGACTCAGAGATCCTCCAGATGTTTGTCCCTTTTGGTCATGTCATCTCAGCCAAAGTCTTTGTTGACC GGGCCACCAATCAGAGCAAATGTTTTGGCTTTGTGAGTTTCGACAATCCGGCCAGTGCCCAGGCTGCCAT CCAGGCTATGAACGGTTTCCAGATTGGCATGAAGCGCCTCAAAGTCCAGCTAAAGCGGCCTAAGGATGCA AACAGGCCCTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289613
Insert Size	1485 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289613.1, NP_001276542.1
RefSeq Size	3136 bp
RefSeq ORF	1485 bp
Locus ID	78784
UniProt ID	Q8CIN6
Gene Summary	RNA-binding protein involved in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.