Wipf1 (NM_001289722) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI115543; D2Ertd120e; Waspip; WIP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228006 representing NM_001289722 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGTCCCTCCCCCACCAGCACCCCCACCGCCACCAACATTTGCACTCGCTAACACTGAGAAGCCCA CCTTGAACAAGACAGAGCAGGCTGGGAGGAATGCTCTTCTCTCTGACATCAGCAAAGGGAAGAAGCTAAA GAAGACGGTCACCAATGACAGAAGTGCCCCAATATTGGACAAACCAAAGGGAGCCGGCGCCAGTGCTGGT GGCTATGGTGGAGGCGGCGGAGGTGGTGGTGGAGGAGGAGGAGGAGGAGGAGGCAGCGGTGGAAATTTTG GAGGGGGTGGTCCTCCAGGACTGGGAGGACTATTCCAGGCTGGAATGCCGAAGCTGAGGTCCACCGCCAA CAGGGATAATGATTCTGGAGGAAGCCGACCACCGATTTTGCCACCGGGAGGAAGAGCCACATCTGCCAAG CCCTTTTCACCCCCGAGTGGCCCGGGGAGGTTTCCCGCACCCTCCCCAGGCCACAGGAGTGGGCCCCCAG AGCCACCCAGGAACAGAATGCCTCCCCCGAGGCCTGACGTGGGCTCAAAACCAGATAGCCTTCCCCCTCC GGTACCAAATACGCCAAGACCTGTTCCGTCGAGTCTGCACAACCGGGGATCCCCAGCAGGGCTGGGAGCC CCCAGGCCCCCCTTTCCCGGGAACCGAGGTGCTGCTTTTGGAGCAGGCTCTGCGCGCCAGAACCCCTCCG GCTCCTCCTCTCCGTTTCCTAGGCCCCCTTTGCCCCCCACCCCAAGCCGAGCCTTGGATGACAAGCCCCC TCCACCACCTCCTCCCGTGGGCAACAGGCCCTCCATGCACAGAGAAGCGGTGCCCCCTCCCCCCTCACAG ACCAGCAAACCTCCAGTGCCTTCCACCCCGCGGCCTGGGTTGGGTTCGCAGGCACCACCTCCTCCTCCAC CGCCCAGCCGGCCAGGGCCCCCTCCTCTGCCTCCAGCTTCCAACGACGAGATCCCCAGGCTGCCACAGCG GAACCTGTCCCTCACGTCCTCTGCACCTCCATTGCCTTCACCTGGACGCTCAGGGCCTCTCCCTCCCCCA CCCAGCGAGAGGCCCCCTCCTCCTGTAAGGGATCCACCAGGCAGATCAGGCCCCCTCCCACCACCACCTC CAATAAACAGAAATGGAAGCACAGCTCGGGCCCTGCCTGCCACCCCTCAGTTGCCATCCAGGAGTGGAAT GGACAGTCCCAGAAGTGGGCCTAGGCCTCCTCTTCCTCCTGACAGGCCTGGTGCTGGGGCGCCGCCCCCA CCCCCACCCTCCACATCAGTTCGAAATGGCTTCCAAGACTCATCGTGTGAAGATGAGTGGGAAAGCAGAT TCTACTTCCATCCGATTTCTGACTTGCCGCCTCCAGAGCCATACGTACCGACAACCAAGACGTATCCCAG CAAACTGGCCAGAAACGAAAGCCGAAGTGGATCCAACAGAAGAGAAAGGGGCGCCCCACCCCTTCCTCCC ATCCCGAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289722
Insert Size	1482 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289722.1, NP_001276651.1
RefSeq Size	4486 bp
RefSeq ORF	1482 bp
Locus ID	215280
UniProt ID	Q8K1I7
Gene Summary	Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation (By similarity). Plays a role in the formation of cell ruffles.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.

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