F10 (NM_001242368) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI1947; Cf10; fX
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228005 representing NM_001242368 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGTCACAGCCACTGCCGTCCTTGACCACACCATGGGGAGCCCGGTGCAACTCAGCCTGCTCTGTG TTGTCCTGGCCAGCCTCCTGCTCCCTGGGAAAGGTGTGTTTATTAACCGGGAACGTGCCAACAATGTCCT GGCGAGGACTCGGAGGGCAAACTCATTTTTTGAAGAGTTCAAGAAAGGAAATCTGGAAAGAGAGTGTATG GAAGAAATTTGTTCTTATGAAGAGGTCCGTGAAATCTTCGAGGACGACGAGAAGACGAAAGAATACTGGA CCAAATATAAAGACGGCGACCAGTGTGAAAGCAGCCCTTGCCAGAACCAAGGAGCGTGTCGAGATGGCAT CGGGGGTTACACGTGCACCTGCTCGGAGGGATTTGAAGGCAAAAACTGTGAGCTCTTTGTTCGGAAACTC TGCCGCCTAGACAACGGAGACTGTGACCAGTTCTGCAGAGAAGAGCAGAACTCAGTGGTGTGCTCCTGCG CCAGCGGTTACTTCCTGGGTAATGATGGCAAGTCTTGCATCTCCACAGCTCCCTTCCCCTGTGGAAAAAT CACTACAGGACGTAGGAAGAGGTCTGTGGCCCTAAACACCAGCGACAGTGAGCTTGACCTTGAAGACGCC CTGCTTGATGAGGATTTCCTGTCCCCTACGGAGAATCCTATTGAACTGCTCAACCTCAACGAGACACAGC CTGAGAGGAGCAGCGATGACCTTGTTCGGATTGTGGGTGGCCGGGAATGCAAGGATGGAGAATGTCCCTG GCAGGCTCTGCTCATTAACGAAGACAATGAAGGGTTCTGTGGGGGCACCATCTTGAATGAGTTCTACATC CTCACTGCTGCCCACTGTCTCCATCAGGCCAGGCGATTCAAGGTGAGGGTAGGTGATCGGAACACAGAGA AGGAAGAAGGCAACGAGATGGTGCACGAGGTGGACGTGGTCATTAAGCACAACAAGTTTCAGAGGGACAC CTACGACTATGATATCGCCGTGCTGAGGCTGAAGACTCCCATCACGTTCCGGATGAACGTGGCCCCTGCC TGCCTGCCTCAGAAAGACTGGGCCGAGTCCACACTGATGACACAGAAGACGGGCATCGTGAGCGGGTTTG GACGCACGCATGAGAAGGGCCGCCAGTCGAACATCCTGAAGATGCTGGAGGTACCCTACGTGGATCGCAA CACCTGCAAGCTCTCCACCAGCTTCAGCATCACACAGAATATGTTCTGTGCGGGCTATGAGGCCAAGTTA GAGGATGCCTGCCAGGGGGACAGTGGTGGCCCCCATGTCACACGGTTCAAAAATACCTACTATGTGACCG GCATTGTCAGCTGGGGAGAGGGGTGTGCAAGGAAAGGGAAATATGGCATCTACACAAAGGTCACGACCTT CCTCAAGTGGATTGACAGGTCCATGAAAGCCAGGGTGGGACCCACAGCCGAGACCCCAAGGACAGCAGGT CCGCCCAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242368
Insert Size	1482 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242368.1, NP_001229297.1
RefSeq Size	2693 bp
RefSeq ORF	1482 bp
Locus ID	14058
Gene Summary	This gene encodes factor X, a component of both the intrinsic and extrinsic blood coagulation pathways. The encoded protein is a zymogen that undergoes further processing in a vitamin K-dependent manner to generate mature factor X, a heterodimer comprised of disulfide-linked heavy and light chains. The mature factor X is proteolytically activated either by factor IXa (intrinsic pathway) or factor VIIa (extrinsic pathway) to form factor Xa serine endopeptidase. Activated factor Xa catalyzes the conversion of prothrombin to thrombin. A complete lack of the encoded protein is fatal to mice. A severe deficiency of the encoded protein in mice causes age-dependent iron deposition and cardiac fibrosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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