Alg11 (NM_001243161) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI849156; AW492253; B230397C21; Mmat-4(5)
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227997 representing NM_001243161 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGACACTGGGAGCTGGTGCGTGTACGCCGTGCTGCGGTTTTTCTATTCACTCTTCTTTCCTG GACTGATGATATGCGGAGTTTTGTGTGTATATTTGGTCATTGGCCTTTGGGTTATCAGGTGGCATCTACA GAGAAAGAAGAAGTCTGTGTCGACCAGCAAAAATGGAAAAGAACAAACGGTGGTTGCATTTTTCCATCCA TACTGTAATGCAGGTGGCGGAGGAGAGAGAGTTCTGTGGTGTGCTTTGAGAGCCCTGCAGAAAAAGTATC CTGAAGCAGTTTATGTTGTATATACTGGTGACATTAATGTCAGTGGTCAACAGATACTAGATGGTGCTTT CAGAAGGTTTAACATCAAGTTGGCTCACCCAGTGCAGTTTGTTTTCTTAAGGAAACGCTACCTTGTGGAA GATTCCCGGTATCCTCACTTCACATTGCTAGGCCAAAGTCTTGGATCCATTCTTCTCGGCTGGGAAGCTC TGATGCAGCGCGTTCCTGATGTTTACATCGACTCAATGGGATACGCCTTCACACTCCCTCTCTTTAAGTA TGTGGGAGGTTGCCGAGTCGGAAGCTATGTTCACTATCCCACGATCAGCACTGACATGCTGTCTGTGGTG AAGAATCAGAATCCTGGATTTAATAATGCTGCTTTCATTAGCAGAAATGCTCTTCTCAGCAAAGCAAAGC TCATCTATTACTATTTATTTGCTTTTGTTTATGGCCTTGTTGGATCTTGCAGTGACATAGTCATGGTTAA TTCTTCTTGGACATTAAACCATATCCTCTCACTGTGGAAGGTTGGGCATTGCACTAACATTGTCTATCCA CCTTGTGATGTGCAGACATTTCTGGACATCCCTTTACATGAGAAGAAGGTGACCCCAGGGCATTTGCTGG TTTCCATTGGCCAGTTCAGACCTGAAAAGAATCATGCTTTGCAGATCAAAGCCTTTGCTAAATTGTTAAA TGAGAAAGCAGCTGAGTTAGGCCATTCTCTTAAACTTGTCCTTATTGGAGGGTGTCGCAACAAAGATGAT GAGTTTAGAGTAAACCAACTGAGAAGTCTATCTGAGAATTTGGGAGTTCAAGAAAATGTGGAATTTAAAA TAAACATTTCATTCGATGAATTGAAGAATTACTTGTCTGAAGCGACCATCGGTCTGCATACTATGTGGAA TGAGCATTTTGGGATTGGAGTTGTCGAGTGTATGGCAGCTGGCACAGTTATCCTTGCACACAACTCAGGA GGCCCGAAGCTGGACATTGTCATTCCTCATGAAGGACAGATAACTGGCTTTCTGGCTGAGAGTGAAGAAG GCTATGCTGACTCTATGGCTCACATTCTTTCTCTGTCTGCAGAAGAGAGACTCCAAATCAGGAAGAATGC TAGAGCGTCTATAAGCAGATTCTCCGACCAGGAGTTTGAAGTAGCATTCCTGTGTTCCATGGAGAAGTTA CTTACTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243161
Insert Size	1479 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243161.1, NP_001230090.1
RefSeq Size	4970 bp
RefSeq ORF	1479 bp
Locus ID	207958
UniProt ID	Q3TZM9
Gene Summary	Mannosyltransferase involved in the last steps of the synthesis of Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum. Catalyzes the addition of the 4th and 5th mannose residues to the dolichol-linked oligosaccharide chain (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1).

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