Tysnd1 (NM_001272091) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1300019N10Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227995 representing NM_001272091 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGGCAATGGGGACCCTCTATGAGAGTGGCGGAGCAGGCGGGCTGCGTGGTGAGCGCCTCGCGGG CCGGACAGCCTGACGCGGGCTCGTGGAGCTGCAGCGGGGTGATTCTGAGCCGTAACCCAGGCCTAGTGCT GTGCCACGGAGGCATCTTTACCCCCTTCCTGCGGACGGGCAGCGCGGCGCTGACCCAGACGGGCACCGCC TTCCTGCCGGGCGACAGTTGCAGCGATGACCTACGTCTCCACGTGCAGTGGGGACCCACGGCTGCCAGCC CTGCCGGTCGCGCGGATCAGGAACTCCCGGGGCTGTGCACGCCTCAGTGCGCAAGCCTGGGCCTCGAACC CGGCGCCCCGAGCCGGGCCCGCGCGCGGCCTCTGCAGCCCCCGCGACCAGCGCAGCTGCTGTTGCTGCTC AGCTGCCCGGCCTTCCGCTCGCATTTCGCTCGGCTCTTCGGGGCCGATGCGGTCGATCAATGGCACTTCG TAAGCTCGGCGCCCGACGATGCGGTTTCGGAGGAGGAAGAGGAGGACCAGCTGCGCGCGCTCGGCTGGTT CGCCCTGCTGCGGGTGCAGCGGGGCGCGGCGGCGGAGGAGAGGCGCGGACCGGTGGTGACCGTGGCACCG CTCGGGGCCGTGGTCAAGGGCGCACCGCTGTTGGCTTGTGGCTCTCCGTTCGGTGCCTTCTGCCCGGACA TCTTCCTCAACACGCTGAGCCGCGGCGTGCTCAGCAACGCGGCCGGGCCGCTGCTGCTCACCGACGCGCG CTGCCTGCCGGGCACGGAAGGCGGCGGAGTGTTCGCCGCTCGACCTGCGGGTGCGCTGGTGGCTCTGGTG GCCGCGCCGCTCTGCTGGAAGGCCCGCGAGTGGGTGGGCCTCACGCTGCTGTGCGCCGCCGCCCCGCTGC TTCAGGTTGCCCGCTGGGCGCTCGCCCGTCTGCACCCCGGCTCCGCTTCCCTGTCTGTGCTGCTGCCGCC ACCCGATGTGAGCACGCCACGGGGCCTGCCTCTGCGCGATCTCGGACCCCCGTGGGCGGCCGCGGCGGTG CTGGTGGAGTGTGGTACCGTGTGGGGCTCCGGAGTGGTCGTGGCGCCTCGCCTGGTGGTCACCTGTCGCC ATGTGGCACCCCGGGAAGCAGCCAGGGTGTTGGTACACTCCGCTACACCCAAGCGAGCCTGTCAGCGTGG TGGGCTTTGGTGTCTTTGGCCAGGCCTGCGGGCCCTCGGTGACCTCAGGCATCCTGTCGGCCGTGGTGCG TGTGGATGGCAGCCCGGTGATGCTACAGACCACGTGCGCTGTGCATGGCGGCTCCAGCGGAGGACCTCTC TTCTCCTCGGGCTCCGGGGACCTCCTGGGCATTGTCGCCAGCAACACTCGAGACAACAACACGGGAGCCA CCTACCCACACCTGAACTTCAGCATCCCCATCACGGTGCTCCAGCCAGCCCTGAAGCAGTACAGTCAGAC TGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001272091
Insert Size	1476 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001272091.1, NP_001259020.1
RefSeq Size	2155 bp
RefSeq ORF	1476 bp
Locus ID	71767
UniProt ID	Q9DBA6
Gene Summary	This gene encodes a protease that removes the N-terminal peroxisomal targeting signal (PTS2) from proteins produced in the cytosol, thereby facilitating their import into the peroxisome. The encoded protein is also capable of removing the C-terminal peroxisomal targeting signal (PTS1) from proteins in the peroxisomal matrix. The full-length protein undergoes self-cleavage to produce shorter, potentially inactive, peptides. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (3) lacks an alternate exon in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (c) is shorter and has a distinct C-terminus, compared to isoform a.

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