Mtf2 (NM_001253878) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9230112N11Rik; AA537621; C76717; M96; Pcl2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227992 representing NM_001253878 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTGTACAATATGTCAAGAAGAGTATTCAGAAGCTCCCAATGAAATGGTTATATGTGATAAGTGTG GTCAAGGATATCATCAGCTGTGTCACACACCTCATATTGACTCGAGTGTGATTGATTCAGATGAAAAGTG GCTTTGTCGACAGTGCGTTTTTGCAACAACTACAAAGAGGGGTGGTGCGCTTAAGAAAGGACCAAATGCC AAAGCATTGCAAGTCATGAAGCAGACATTACCCTATAGTGTGGCTGACCTTGAATGGGATGCAGGCCATA AAACCAATGTCCAGCAGTGCTACTGCTATTGTGGAGGCCCTGGAGACTGGTATTTAAAGATGCTGCAGTG CTGCAAATGTAAGCAGTGGTTTCATGAAGCTTGTGTCCAATGCCTTCAGAAGCCAATGCTGTTTGGAGAT AGGTTTTATACATTTATTTGCTCTGTCTGCAGTTCTGGACCAGAATACCTCAAACGTCTACCGTTACAGT GGGTAGATATAGCACACCTATGCCTTTACAACCTAAGTGTTATTCACAAGAAGAAATACTTTGATTCTGA ACTTGAGCTTATGACATACATTAATGAAAACTGGGATAGATTGCACCCTGGAGAGCTGGCAGACACACCC AAATCTGAAAGATATGAGCATGTTCTGGAGGCATTAAATGATTACAAGACCATGTTTATGTCTGGGAAAG AAATAAAGAAGAAGAAGCATTTGTTTGGGTTGCGAATTCGTGTTCCTCCTGTGCCACCAAATGTGGCTTT CAAAGCAGAGAAAGAACCAGAAGGAACATCCCATGAATTTAAAATTAAAGGCAGAAAGGCATCCAAACCT ACATCTGACTCAAGGGAAGTAAGCAATGGGATAGAAAAAAAAGGAAAGAAAAAATCTGTAGGTCGTCCTC CTGGCCCATATACAAGAAAAATGATTCAAAAAACTGCTGAGCTACCTTTGGATAAGGAATCAGTTTCAGA GAATCCTACGTTGGATTTACCTTGTTCTATAGGGAGAACTGAGGGAATTGCACATTCATCCAATACCTCA GATGTGGACCTCACGGGTGCTTCCAGTGCAAACGAAACTACCTCGGCTAGCATTTCCAGGCATTGTGGAT TATCAGACTCTAGAAAAAGAACTCGCACAGGAAGATCATGGCCTGCTGCAATACCACATTTGCGGAGAAG AAGAGGTCGCCTTCCACGAAGAGCACTCCAGACTCAGAACTCAGAAGTTGTGAAAGATGATGAAGGCAAA GAAGATTATCAGTTTGAAGAACTTAACACAGAAATTCTGAATAACTTAGCAGATCAGGAGTTGCAGCTCA ATCACCTGAAAAACTCCATCACTAGTTATTTTGGTGCCGCAGGTAGAATAGCATGTGGCGAAAAATACAG AGTTTTGGCTCGTCGGGTGACGCTTGATGGGAAGGTGCAGTATCTTGTGGAATGGGAAGGAGCGACTGCA TCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253878
Insert Size	1476 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253878.1, NP_001240807.1
RefSeq Size	4424 bp
RefSeq ORF	1476 bp
Locus ID	17765
UniProt ID	Q02395
Gene Summary	Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element (MRE) of MT1A gene promoter.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 7, which results in the use of a downstream AUG start codon. The resulting isoform (2) is shorter at the N-terminus compared to isoform 3. Variants 2-6 all encode the same isoform (2).

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