Gpcpd1 (NM_001291050) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310004G06Rik; 2310032D16Rik; AU015213; Gde5; mKIAA1434; Prei4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227986 representing NM_001291050 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAACAGCCTGGAGATATCCTTAATAAGTGACAATGAGTTCAAGTGCAGGCACTCACAGCCAGAAT GTGGGTATGGCTTACAGCCCGATCGTTGGACAGAGTACAGCATACAGACAATGGAACCAGATAATCTGGA GCTCATCTTTGACTTTTTTGAGGAAGATCTCAGTGAGCATGTAGTTCAGGGTGATGTTCTTCCTGGACAC GTGGGCACAGCATGCCTCCTGTCTTCTACCATTGCTGAGAGTGGAAGAAGCGCTGGAATCCTTACTCTTC CCATCATGAGCAGAAATTCCAGAAAAACTATAGGCAAAGTCAGAGTTGATTTTATCATCATCAAGCCATT ACCTGGATATAGTTGTTCTATGCAGTCTTCATTTTCCAAGTATTGGAAACCAAGAATACCATTGGACGTT GGACATCGTGGTGCAGGGAACTCAACAACGACTGCCAAGCTAGCTAAAGTACAGGAAAATACTATCGCTT CTTTAAGAAATGCTGCCAGTCATGGCGCAGCATTTGTAGAATTTGATGTCCACCTTTCAAAGGACTTTGT GCCCGTGGTGTATCATGACCTCACCTGCTGTCTGACCATGAAGAGGAAATATGAAGCTGATCCAGTTGAA TTGTTTGAAATCCCAGTAAAAGAATTAACATTTGACCAACTCCAGTTATTGAAGCTTTCTCATGTGACTG CATTAAAAACCAAAGACCGGAAACAATCTTTGTATGAGGAGGAAAATTTCTTTTCTGAAAATCAGCCATT TCCTTCTCTTAAGATGGTTTTAGAATCATTGCCAGAAAATGTAGGATTTAATATAGAAATAAAATGGATT TGCCAACACAGGGATGGAGTATGGGATGGCAACTTATCAACATATTTTGATATGAATGTGTTTTTGGATA TAATTTTAAAAACTGTTTTAGAAAATTCTGGGAAGAGGAGAATAGTGTTTTCTTCTTTTGATGCAGATAT TTGTACAATGGTTCGGCAGAAGCAGAACAAATATCCCATATTATTTTTGACCCAAGGAAAGTCTGATATT TACCCCGAACTCATGGACCTCAGATCTCGGACAACACCCATTGCAATGAGTTTTGCACAGTTTGAAAATA TTTTGGGGATAAATGCCCATACTGAAGACCTCCTTAGAAACCCATCCTATGTCCAAGAGGCAAAAGCTAA GGGATTGGTCATATTCTGCTGGGGTGATGATACCAACGATCCTGAAAACAGAAGGAAACTGAAGGAATTT GGAGTAAATGGTCTAATATATGATAGGATATATGATTGGATGCCTGAACAGCCAAATATATTCCAAGTGG AGCAGTTGGAGCGCCTGAAGCAAGAATTGCCAGAGCTTAAGAACTGTTTGTGTCCCACTGTTAGCCACTT CATCCCTTCTTCTTTCTGTGTGGAGCCTGATATCCACGTGGATGCCAACGGCATTGATAGTGTGGAGAAC GCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291050
Insert Size	1476 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291050.1, NP_001277979.1
RefSeq Size	3592 bp
RefSeq ORF	1476 bp
Locus ID	74182
UniProt ID	Q8C0L9
Gene Summary	May be involved in the negative regulation of skeletal muscle differentiation, independently of its glycerophosphocholine phosphodiesterase activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence and lacks an in-frame exon compared to variant 8. The resulting isoform (1) is shorter at the N-terminus and lacks an alternate internal segment compared to isoform 4. Variants 1, 5, 6, 7, and 12 all encode the same isoform (1).

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