Cbll1 (NM_001253847) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI467391; c-Cbl-like; Hakai
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227985 representing NM_001253847 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCACACTGACAATGAGTTACAAGGCACTAATAGTTCTGGATCCTTGGGTGGTCTTGATGTTCGCA GAAGAATCCCTATAAAGCTCATCTCCAAACAAGCCAGCAAAGTTAAGCCGGCACCTCGGACTCAAAGGAC TGTCAGCAGGATGCCCGCAAAGGCCCCGCAAGGTGATGAAGAAGGATTTGATTATAACGAAGAACAGCGA TATGACTGTAAAGGAGGCGAACTCTTTGGGAATCAGCGAAGATTTCCAGGACACCTTTTTTGGGATTTCA AGATAAACATCTTAGGTGAAAAGGACGATACACCAGTACATTTCTGTGACAAATGTGGACTGCCTATTAA AGTCTATGGGAGAATGATTCCATGCAAGCATGTCTTTTGCTATGACTGTGCTATTTTACATGAAAAAAAA GGAGATAAGATGTGCCCAGGATGTAGTGATCCTGTGCAGCGGATTGAGCAGTGCACACGAGGTTCTCTCT TTATGTGTAGCATTGTTCAAGGATGCAAGAGAACATATCTGTCTCAGAGAGACTTACAAGCTCATATCAA CCATCGCCATATGAGAGCTGGAAAGCCCGTTACCCGTGCTTCACTTGAGAATGTTCATCCTCCTATTGCC CCCCCACCAACTGACATCCCCGATCGGTTCATAATGCCACCAGACAAGCATCATATGAGCCATATTCCTC CAAAGCAGCACATCATGATGCCACCGCCTCCTCTGCAGCATGTGCCACATGAGCACTATAATCAGCCACA TGAGGATATTCGTGCTCCTCCGGCAGAATTGTCCATGGCTCCACCTCCACCTCGTTCGGTCAGTCAGGAA ACCTTTCGTATTTCAACAAGAAAACACAGCAATTTAATAACGGTCCCTATTCAGGATGACTCCAGTTCAG GTGCTAGAGAACCACCACCTCCTGCCCCAGCACCTGCTCATCACCATCCTGAATATCAGGGTCAGCCCGT GGTATCTCACCCTCATCATATTATGCCTCCACAGCAACATTACGCACCACCCCCACCTCCTCCACCACCA ATAAGCCATCCAATGCCACACCCTCCCCAGGCTGCAGGTACTCCTCACTTGGTATATAGCCAAGCTCCAC CTCCACCAATGACCTCTGCTCCACCCCCAATAACCCCTCCCCCTGGACATATTATTGCCCAGATGCCACC TTATATGAATCATCCTCCTCCAGGACCCCCTCCACCTCAGCATGGTGGTCCACCTGTAACTGCACCCCCT CCTCACCATTACAATCCTAACTCTTTACCCCAGTTTACTGAAGACCAAGGAACTCTGAGCCCTCCATTTA CACAACCAGGAGGAATGAGTCCTGGTATATGGCCTGCACCAAGAGGGCCACCTCCTCCTCCACGAATGCA GGGCCCGCCTTCTCAAACCCCACTACCTGGACCGCATCATCCAGATCAAACAAGATACAGACCGTATTAC CAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253847
Insert Size	1476 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253847.1, NP_001240776.1
RefSeq Size	4007 bp
RefSeq ORF	1476 bp
Locus ID	104836
UniProt ID	Q9JIY2
Gene Summary	E3 ubiquitin-protein ligase that mediates ubiquitination of several tyrosine-phosphorylated Src substrates, including CDH1, CTTN and DOK1 (PubMed:11836526, PubMed:22252131). Targets CDH1 for endocytosis and degradation (PubMed:11836526). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29535189, PubMed:29547716). Its function in the WMM complex is unknown (PubMed:29535189, PubMed:29547716).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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