Rabgef1 (NM_001199059) Mouse Untagged Clone

CAT#: MC227983

Rabgef1 (untagged) - Mouse RAB guanine nucleotide exchange factor (GEF) 1 (Rabgef1), transcript variant 2



  "NM_001199059" in other vectors (1)

CNY 4,660.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Rab5ef; Rabex5; Rin2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227983 representing NM_001199059
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCTGAAGTCCGAACGCAGGGGAATTCATGTGGATCAATCTGAGCTCCTGTGCAAGAAAGGATGCG
GTTACTACGGCAACCCTGCCTGGCAGGGTTTCTGCTCCAAGTGCTGGAGGGAGGAGTACCACAAGGCCCG
GCAGAGGCAGATCCAAGAGGACTGGGAACTGGCAGAACGACTTCAGCGGGAGGAGGAAGAGGCCTTCGCG
AGCAGCCAGAGCAGCCAAGGAGCCCAGTCCCTCACCTTCTCCAAGTTCGAGGAGAAGAAGACCAATGAGA
AAACCCGAAAAGTCACCACAGTGAAGAAGTTCTTCAGCGCCTCTTCCAGAGCTGGATCCAAGAAGGAAAT
TCAGGAAGCCAAAGCTCCCAGTCCCTCCATAAACCGGCAAACCAGCATTGAGACGGACCGAGTGACTAAG
GAGTTCATAGACTTTCTCAAGACCTTCCACAAGACAGGCCAAGAAGTCTATAAACAGACGAAGATGTTTT
TGGAAGCAATGCCTTATAAAAGGGATTTAAGCATCGAGGAACAGTCAGAATGTACTCAGGACTTTTACCA
AAATGTGGCTGAAAGAATGCAGACCCGTGGGAAAGTGCCTCCAGAGAAAGTGGAGAAGATAATGGATCAG
ATCGAAAAGCACATCATGACGCGTCTCTATAAATTTGTGTTCTGCCCAGAGACTACTGATGATGAGAAGA
AAGATCTCGCCATTCAAAAAAGAATCAGGGCCCTGCACTGGGTAACGCCTCAGATGCTCTGTGTCCCTGT
CAATGAGGAAATCCCTGAAGTGTCCGACATGGTGGTGAAAGCGATCACAGACATCATTGAGATGGACTCA
AAGCGTGTGCCTCGGGACAAGCTGGCCTGCATCACCAGGTGCAGCAAGCACATCTTCAATGCCATCAAGA
TCACCAAGAATGAGCCAGCCTCTGCCGATGACTTCCTGCCCACCCTGATCTACATCGTCCTGAAGGGCAA
CCCCCCTCGCCTGCAGTCCAACATCCAGTACATCACTCGCTTCTGCAACCCCAGCCGGCTCATGACGGGC
GAGGATGGCTACTACTTCACCAACCTGTGCTGTGCTGTGGCTTTCATTGAGAAATTAGACGCCCAGTCTT
TGAATTTAAGTCAGGAGGATTTTGACCGGTACATGTCTGGCCAGACATCCCCCAGGAAGCAGGAGTCTGA
GAGTTGGCCCCCGGAGGCCTGCTTAGGTGTGAAGCAAATGTATAAGAACTTGGACCTCCTGTCTCAGTTG
AATGAACGGCAAGAAAGGATCATGAACGAAGCCAAGAAACTTGAAAAAGACTTAATAGACTGGACAGACG
GGATTGCCAAGGAAGTTCAAGACATTGTTGAGAAATACCCACTGGAGATTAAGCCCCCGAACCAACCCTT
AGCAGCCATCGACTCTGAGAATGTGGAGAACGACAAGCTCCCTCCCCCTCTGCAGCCTCAGGTGTACGCA
GGGTGA


ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-NotI     
ACCN NM_001199059
Insert Size 1476 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001199059.1, NP_001185988.1
RefSeq Size 2698 bp
RefSeq ORF 1476 bp
Locus ID 56715
UniProt ID Q9JM13
Gene Summary Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A (By similarity). Can act as a ubiquitin ligase (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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