Rabgef1 (NM_001199059) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Rab5ef; Rabex5; Rin2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227983 representing NM_001199059 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTGAAGTCCGAACGCAGGGGAATTCATGTGGATCAATCTGAGCTCCTGTGCAAGAAAGGATGCG GTTACTACGGCAACCCTGCCTGGCAGGGTTTCTGCTCCAAGTGCTGGAGGGAGGAGTACCACAAGGCCCG GCAGAGGCAGATCCAAGAGGACTGGGAACTGGCAGAACGACTTCAGCGGGAGGAGGAAGAGGCCTTCGCG AGCAGCCAGAGCAGCCAAGGAGCCCAGTCCCTCACCTTCTCCAAGTTCGAGGAGAAGAAGACCAATGAGA AAACCCGAAAAGTCACCACAGTGAAGAAGTTCTTCAGCGCCTCTTCCAGAGCTGGATCCAAGAAGGAAAT TCAGGAAGCCAAAGCTCCCAGTCCCTCCATAAACCGGCAAACCAGCATTGAGACGGACCGAGTGACTAAG GAGTTCATAGACTTTCTCAAGACCTTCCACAAGACAGGCCAAGAAGTCTATAAACAGACGAAGATGTTTT TGGAAGCAATGCCTTATAAAAGGGATTTAAGCATCGAGGAACAGTCAGAATGTACTCAGGACTTTTACCA AAATGTGGCTGAAAGAATGCAGACCCGTGGGAAAGTGCCTCCAGAGAAAGTGGAGAAGATAATGGATCAG ATCGAAAAGCACATCATGACGCGTCTCTATAAATTTGTGTTCTGCCCAGAGACTACTGATGATGAGAAGA AAGATCTCGCCATTCAAAAAAGAATCAGGGCCCTGCACTGGGTAACGCCTCAGATGCTCTGTGTCCCTGT CAATGAGGAAATCCCTGAAGTGTCCGACATGGTGGTGAAAGCGATCACAGACATCATTGAGATGGACTCA AAGCGTGTGCCTCGGGACAAGCTGGCCTGCATCACCAGGTGCAGCAAGCACATCTTCAATGCCATCAAGA TCACCAAGAATGAGCCAGCCTCTGCCGATGACTTCCTGCCCACCCTGATCTACATCGTCCTGAAGGGCAA CCCCCCTCGCCTGCAGTCCAACATCCAGTACATCACTCGCTTCTGCAACCCCAGCCGGCTCATGACGGGC GAGGATGGCTACTACTTCACCAACCTGTGCTGTGCTGTGGCTTTCATTGAGAAATTAGACGCCCAGTCTT TGAATTTAAGTCAGGAGGATTTTGACCGGTACATGTCTGGCCAGACATCCCCCAGGAAGCAGGAGTCTGA GAGTTGGCCCCCGGAGGCCTGCTTAGGTGTGAAGCAAATGTATAAGAACTTGGACCTCCTGTCTCAGTTG AATGAACGGCAAGAAAGGATCATGAACGAAGCCAAGAAACTTGAAAAAGACTTAATAGACTGGACAGACG GGATTGCCAAGGAAGTTCAAGACATTGTTGAGAAATACCCACTGGAGATTAAGCCCCCGAACCAACCCTT AGCAGCCATCGACTCTGAGAATGTGGAGAACGACAAGCTCCCTCCCCCTCTGCAGCCTCAGGTGTACGCA GGGTGA ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_001199059
Insert Size	1476 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199059.1, NP_001185988.1
RefSeq Size	2698 bp
RefSeq ORF	1476 bp
Locus ID	56715
UniProt ID	Q9JM13
Gene Summary	Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A (By similarity). Can act as a ubiquitin ligase (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.