Agfg2 (NM_001303266) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A630095P14Rik; Hr; Hrbl; RAB-R; RABR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227979 representing NM_001303266 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGATGGCGGCTAAGAAGGGCCCGGGCCCGGGTGGCGGGGTCGGAGGGAGCAAAGCGGAGGCTGAAG CCGCTTCGGAGGTGTGGTGCCGCCGAGTGCGGGAGCTGGGCGGCTGCAGCCAGGCCGGGAACCGCCACTG TTTCGAGTGCGCCCAGCGCGGGGTCACGTATGTGGACATCACCGTGGGCAGCTTCGTCTGCACCACCTGC TCCGGCCTCCTGAGAGGCCTGAACCCCCCTCATCGAGTCAAGTCAATCTCCATGACAACTTTCACTGAGC CTGAAGTCCTGTTCCTCCAATCTCGTGGAAATGAGGTCTGTCGGAAAATCTGGCTCGGTCTTTTTGATGC TCGGACATCGTTGATACCAGATTCCAGGGATCCTCAGAAGGTGAAGGAGTTTCTCCAAGAAAAATATGAG AAAAAAAGATGGTACGTCCCCCCAGAGCAAGTCAAGGGGCCCTCTTACAGCAAAGGCAGTGTCTCTGCTA CCCCTGTCCAGGGCTCTGTCCCAGAAGGGAAACCCATTCGGACACTTCTGGGAGACCCTGTGCCATCTCT CTCTGATCCTGCTTCCACTTCAAGCCAGCCTGGGAGCCAGTCGCAGGCACGCAGCAGCTCGCAGGCCAGG AGCTCCCAGCCTCCTTCCCATTCATCCACCAAGAAAGCCAGCACTGACCTGCTGGCGGATATCGGGGGAG ACCCCTTTGCTGCTCCCCAGGTGGTGCCAGCCTTTGCCTCATTCCCAGGCTTTGGAGTAGGCCAGACTCC TGCCCATGGAGGCTTTGCCAACTTCGATGCCTTCAGCAGCAGCCCTAGCTCTTCCACCTTCGGAAGCCTC CCTCCATCCGTCCAAGCGCCATTCCAGGCCCAGCCGACCCCTGCAGCCAATCGGATGCTAACTGGAAGTT ACAGCTTTGGAAGTGGCCAGATGTCTGCGTTTGGTGTGGCACCCCTTGCAGCTGCCAGTCAACCCAACAA CCTTGCAGATGTGGGCGGCCTCCTGGGTCCCAGGATGGCTGCTGGAGGTCTCCCTGGCAGTGTTTTTGGG ATGCCGAGCCAGGTTCCTGCCCTGCAGTCGGCCGTGCCAGGTGTTAGCGGCAGTGGAGGGCTCCCCTTTG GAGCCTACACCAACCCCTTCGCCACCCCTGCCCAAGCCCAGCTGCCTTCTACCAACCCATTCCAACCCAA TGGTCTAGCCTCAGGGCCTGGCTTTGGGATGAGCAGTGTTCGGCCTGGCCTTCTCCAGCCAGTGCCACCC TCCGGGGCCTTTGCCAGTCCCTTCTCTGCACCCGTGTTCCCCACACAGGCTGGACTGGCCGACCAGCAGA ATGGATCTTCCTTTGGCGACTTGGGGACCTCTAAGCTGGGGCAGAGGCCACTGAGCCAGCCGGCTGGGAT CTCTACCAATCCTTTCATGACTGGATCCTCAGCGTTTGCCTCCAAACCTCCAACCACAAACCCATTCTTG TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001303266
Insert Size	1473 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303266.1, NP_001290195.1
RefSeq Size	2953 bp
RefSeq ORF	1473 bp
Locus ID	231801
Gene Summary	This gene encodes a paralog of the HIV-1 Rev binding proteins that serve as cellular co-factors for HIV-1 Rev protein in shuttling viral pre-mRNAs from the nucleus to the cytoplasm. The encoded protein contains an ADP-ribosylation factor GTPase activating protein (Arf-GAP) zinc finger domain, several phenylalanine-glycine (FG) motifs and asparagine-proline-phenylalanine (NPF) motifs. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (3) contains an additional in-frame exon in the 3' coding region, compared to variant 2. The resulting protein (isoform 3) is longer than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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