Hrh1 (NM_001252643) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Bphs; H1R; HH1R; Hir
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227966 representing NM_001252643 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTTCCCAACACCTCTTCTGCCTCCGAAGACAAGATGTGTGAGGGGAACAGGACAGCCATGGCCA GCCCTCAGCTGCTGCCCCTGGTGGTGGTTCTAAGTAGTATCTCCCTGGTCACAGTGGGCCTCAACCTGCT GGTGCTGTATGCAGTGCGCAGTGAGCGCAAGCTACACACCGTGGGCAACCTGTACATTGTCAGCCTGTCG GTAGCAGACCTGATTGTAGGGGCAGTCGTCATGCCCATGAACATCCTCTATCTTATCATGACCAAGTGGT CCCTGGGCCGCCCCCTCTGCCTCTTTTGGCTCTCTATGGATTATGTGGCCAGCACGGCATCCATCTTTAG TGTCTTCATCCTGTGTATTGATCGCTACCGCTCTGTCCAGCAACCCCTCCGGTACCTGAGGTATCGAACC AAGACCCGTGCTTCAGCTACCATCCTGGGGGCCTGGTTTCTCTCCTTCCTGTGGGTTATACCTATACTTG GCTGGCATCACTTCACGCCCCTGGCCCCAGAGCTTCGGGAAGATAAGTGTGAGACAGATTTCTACAATGT CACTTGGTTCAAGATCATGACCGCCATCATCAACTTCTACCTCCCCACTTTGCTCATGCTGTGGTTCTAT GTGAAGATCTACAAGGCTGTGCGGCGACACTGTCAGCACCGCCAGCTCACCAACGGGTCCCTCCCTACCT TTTTAGAAATCAAGCTGAGGTCGGAGGATGCCAAAGAGGGTGCCAAGAAACCTGGGAAAGAGTCTCCCTG GGGGGTCCAGAAGAGGCCGTCAAGAGACCCTACTGGAGGTCTGGATCAGAAGTCAACATCTGAAGACCCC AAGGTGACCTCTCCGACTGTCTTCAGCCAAGAGGGGGAAAGGGAAACAGTCACACGCCCCTGTTTCCGTC TTGACGTCATGCAGACACAGCCTGTGCCTGAGGGAGATGCCAGGGGCTCAAAGGCCAATGACCAGACCTT GAGCCAGCCCAAAATGGATGAGCAGAGCCTGAGTACTTGCCGGCGGATCAGTGAGACATCAGAGGACCAG ACCTTGGTGGATCGACAGTCCTTCTCCCGGACCACAGACTCAGACACCAGCATAGAGCCAGGGCTGGGCA AAGTCAAAGCGAGAAGCAGGTCTAACAGTGGCCTGGACTACATCAAAGTCACCTGGAAGAGGCTCCGCTC ACATTCCAGACAGTATGTGTCCGGGTTGCACTTGAACCGAGAGCGGAAGGCAGCCAAGCAGTTGGGTTGT ATCATGGCAGCATTCATTCTCTGCTGGATTCCCTATTTCATCTTCTTCATGGTCATTGCCTTCTGCAACA GCTGCTGCAGCGAACCTGTGCACATGTTCACCATTTGGCTGGGCTACATCAACTCCACGCTGAACCCCCT CATCTACCCGCTGTGCAACGAGAACTTCAAGAAGACATTCAAAAAAATTCTGCACATTCGTTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252643
Insert Size	1467 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252643.2, NP_001239572.1
RefSeq Size	3870 bp
RefSeq ORF	1467 bp
Locus ID	15465
UniProt ID	P70174
Gene Summary	In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. Involved in circadian rhythm of locomotor activity and exploratory behavior. Also involved in responsiveness to pertussis toxin through its control of susceptibility to histamine hypersensitivity and enhancement of antigen-specific delayed-type hypersensitivity responses.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 6. Variants 1 thorugh 6 encode the same protein.

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