Celf1 (NM_001244891) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1600010O03Rik; AA407467; Brunol2; CUG-BP; CUG-BP1; CUGBP; Cugbp1; D2Wsu101e; HNAB50; NAB50
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227944 representing NM_001244891 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGGCACCCTGGACCACCCAGACCAACCAGATCTTGATGCTATCAAGATGTTTGTGGGCCAGGTTC CAAGGACCTGGTCTGAGAAAGACTTGAGGGAGCTTTTTGAGCAGTATGGTGCTGTCTATGAAATCAACAT CCTAAGGGATAGGAGCCAAAACCCTCCTCAGAGCAAAGGGTGCTGTTTTGTTACATTTTACACCCGTAAA GCTGCATTAGAAGCTCAGAATGCTCTCCATAACATGAAGGTCCTCCCTGGGATGCATCACCCTATACAGA TGAAGCCTGCTGACAGTGAAAAGAACAATGCTGTGGAAGACAGGAAGCTCTTTATTGGTATGATTTCCAA GAAGTGTACTGAAAATGACATCCGAGTCATGTTTTCTTCGTTTGGACAGATTGAAGAGTGCCGGATATTG CGGGGACCTGATGGCTTGAGCAGAGGTTGTGCATTTGTGACTTTTACTACAAGAACCATGGCACAGACAG CTATCAAAGCAATGCACCAAGCACAAACCATGGAGGGTTGCTCATCCCCAATGGTGGTAAAGTTTGCCGA CACCCAGAAGGACAAAGAACAGAAGAGAATGGCCCAGCAGCTCCAGCAGCAGATGCAGCAGATCAGTGCA GCGTCTGTGTGGGGAAACCTGGCTGGTCTGAACACACTTGGACCCCAGTACTTAGCACTTTATTTGCAGC TCCTACAGCAGACTGCCTCCTCTGGGAACCTCAACACCCTGAGCAGCCTCCACCCAATGGGAGGGTTAAA TGCAATGCAGCTACAGAATTTGGCTGCACTAGCTGCTGCAGCTAGTGCAGCTCAGAACACACCAAGTGGT ACCAATGCTCTCACTACATCCAGCAGTCCCCTCAGCGTACTCACCAGTTCAGGGTCTTCACCGAGCTCCA GCAGCAGTAATTCTGTCAACCCCATAGCCTCACTTGGAGCCCTGCAAACATTGGCCGGAGCAACAGCTGG CCTCAACGTTGGCTCATTGGCAGGGATGGCTGCTTTGAATGGTGGTCTGGGCAGCAGTGGCCTTTCCAAT GGCACTGGGAGTACCATGGAAGCCCTCACCCAGGCGTATTCTGGTATCCAGCAATATGCTGCTGCAGCCC TCCCCACTCTGTACAACCAGAATTTATTGACACAGCAGAGTATTGGTGCTGCTGGAAGCCAGAAGGAAGG TCCAGAAGGAGCCAACCTGTTCATCTATCACCTGCCCCAGGAGTTTGGAGACCAGGACTTACTGCAGATG TTTATGCCCTTTGGGAATGTCGTGTCGGCCAAGGTTTTCATAGACAAGCAGACAAACCTGAGCAAGTGCT TTGGTTTTGTAAGTTATGACAATCCTGTCTCAGCTCAAGCTGCCATCCAGTCCATGAACGGCTTTCAAAT TGGAATGAAGAGGCTTAAAGTGCAGCTCAAACGTTCGAAGAATGATAGTAAGCCCTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001244891
Insert Size	1461 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001244891.1, NP_001231820.1
RefSeq Size	7849 bp
RefSeq ORF	1461 bp
Locus ID	13046
UniProt ID	P28659
Gene Summary	RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing (By similarity). Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition (By similarity). Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs (By similarity). Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB (By similarity). Promotes exclusion of exon 11 of the INSR pre-mRNA (By similarity). Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast (By similarity). Increases translation and controls the choice of translation initiation codon of CEBPB mRNA (By similarity). Increases mRNA translation of CEBPB in aging liver. Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3 (By similarity). Mediates rapid cytoplasmic mRNA deadenylation (By similarity). Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation (By similarity). Required for completion of spermatogenesis. Binds to (CUG)n triplet repeats in the 3' UTR of transcripts such as DMPK and to Bruno response elements (BREs) (By similarity). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity). Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3' UTR of JUN and FOS mRNAs. Binds to the IR RNA (By similarity). Binds to the 5'-region of CDKN1A and CEBPB mRNAs (By similarity). Binds with the 5'-region of CEBPB mRNA in aging liver. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF2, negatively regulates the processing to mature miRNA (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has an alternate exon in the 5' region, which results in a downstream AUG start codon, and has an additional segment in the 3' UTR, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.