Usp3 (NM_001302116) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA409661; BC017156
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227931 representing NM_001302116 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGTCCGCACCTCAGCTCCAGCGTCTGCATCGCCCCGGACTCAGCCAAGTTCCCCAACGGCTCCC CGTCGTCCTGGTGCTGCAGCGTGTGCAGGTCCAACAAAAGCCCCTGGGTCTGTTTGACTTGTTCAAGTGT CCACTGTGGAAGGTATGTGAATGGCCATGCAAAAAAACATTATGAAGATGCACAGATACCCTTACTCAAC CATAAGAGATCAGAAAAGCAGGAGAAAGCTCAGCATACAGTGTGTATGGATTGCAGTAGCTACAGCACAT ACTGTTATCGCTGTGACGATTTTGTGGTCAACGACACCAAGCTGGGCCTGGTACAGAAAGTGAGAGAACA CTTACAGAACTTGGAAAACTCAGCCTTTACAGCGGACAGGCACAGAAAAAGAAAATTATTGGAAAACTCA TCACTAAACAGCAAGTTATTAAAAGTAAATGGAAGTACGACTGCCATTTGTGCCACAGGCCTTCGAAATT TGGGCAACACTTGTTTCATGAATGCTATCCTTCAGTCACTCAGGTCTTTAGTAGAAGAGTTTAGAAAGAC ACTGTGTGCTTTATGGCAAGGCAGCCAGACTGCATTTAGTCCTGAGTCCTTGTTTTATGTTGTCTGGAAG ATCATGCCTAACTTTAGGGGTTATCAGCAGCAGGATGCCCATGAATTCATGCGCTACCTTTTGGATCATC TACACTTAGAACTTCAGGGTGGCTTCAATGGTGTTTCCCGCTCAGCAATTCTACAGGAGAATTCTACACT GTCTGCAAGTAACAAATGCTGCATAAATGGAGCATCAACTGTTGTCACAGCAATATTTGGAGGAATTCTC CAGAACGAGGTCAACTGCCTCATATGTGGGACAGAGTCGAGGAAGTTTGATCCATTCCTAGATCTCTCCT TAGATATTCCAAGTCAGTTCAGAAGCAAGCGCTCTAAGAATCAGGAAAATGGGCCAGTTTGCTCCTTAAG AGATTGCCTTCGCAGCTTCACTGACCTAGAGGAACTTGATGAGACAGAGTTGTATATGTGCCATAAGTGC AAAAAGAAACAAAAGTCCACAAAGAAGTTTTGGATTCAAAAACTTCCCAAGGCACTGTGCTTGCACCTGA AAAGGTTTCACTGGACAGCATATTTAAGAAACAAAGTCGATACATACGTTCAATTCCCACTGAGAGGCCT GGACATGAAGTGCTACCTACTAGAGCCGGAGAACAGTGGTCCAGACAGTTGCCTGTATGACCTCGCTGCT GTGGTTGTACACCATGGCTCTGGGGTTGGTTCTGGACATTACACAGCATATGCAGTTCACGAAGGCCGCT GGTTCCATTTCAATGACAGCACTGTGACAGTGACTGATGAGGAGACCGTTGGGAAGGCAAAGGCCTACAT TCTGTTTTATGTGGAGCGTCAGGCCAGAGCTGGAGCAGAAAAACTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302116
Insert Size	1449 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302116.1, NP_001289045.1
RefSeq Size	5480 bp
RefSeq ORF	1449 bp
Locus ID	235441
Gene Summary	The protein encoded by this gene is a chromatin-associated histone 2A and 2B deubiquitinating enzyme that negatively regulates the DNA damage response. Mice deficient for this enzyme have reduced hematopoietic stem cell reserves, demonstrating a requirement in hematopoietic stem cell homeostasis. In addition, knock down of protein levels results in spontaneous tumor development and shortened lifespan, consistent with a function in preserving chromosomal integrity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region. This results in a shorter protein (isoform 2), compared to isoform 1.

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