Rarb (NM_001289760) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A830025K23; Hap; Nr1b2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227929 representing NM_001289760 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCACCAGCAGCCACGCATGCCCAGTCCCGGCAGTGCGTGGACACATGACTCACTACCCAGCTGCAC CCTACCCATTACTGTTCCCCCCTGTCATCAGAGGACTCTCCCTGCCTCCACTCCATGGGCTCCATGGCCA CCCACCTCCCAGTGGATGCAGCACCCCATCACCAGCCAGTGTGGGGCAGGCATGTCAGAGGACAACTGGT GGAAGTCAGTTTGCAGCTTCTACCAAGTGGACACCAAGCTTGAATGCAGCCATCGAGACACAGAGTACCA GCTCTGAGGAGCTCGTCCCGAGCCCACCATCTCCACTTCCTCCTCCTCGGGTGTACAAGCCCTGCTTCGT TTGCCAGGACAAGTCATCGGGCTACCACTATGGCGTCAGTGCCTGCGAGGGGTGCAAGGGCTTTTTCCGC AGAAGTATTCAGAAGAACATGATCTACACTTGCCATCGAGATAAGAACTGCGTCATTAACAAGGTCACTA GGAACCGATGCCAGTACTGCCGCCTGCAGAAGTGCTTTGAAGTGGGCATGTCCAAAGAGTCTGTTAGGAA TGACAGGAACAAGAAAAAGAAGGAGCCTTCAAAGCAGGAATGCACAGAGAGCTATGAGATGACAGCGGAG CTAGACGACCTCACTGAGAAGATCCGGAAAGCCCACCAGGAAACCTTTCCCTCACTCTGCCAGCTGGGTA AATACACCACGAATTCCAGCGCTGACCACCGGGTCCGATTGGACTTGGGCCTCTGGGACAAATTCAGTGA GCTGGCCACCAAGTGCATTATTAAGATCGTGGAGTTCGCCAAGCGTCTGCCGGGCTTCACAGGTCTGACC ATCGCAGACCAGATCACCCTGCTCAAAGCCGCCTGCTTGGATATCTTGATTCTCAGAATTTGTACCAGGT ATACCCCAGAGCAAGACACCATGACTTTCTCTGATGGCCTTACACTAAATCGAACTCAGATGCACAATGC TGGCTTCGGTCCTCTGACTGACCTTGTGTTCACCTTTGCCAACCAGCTCCTGCCTTTGGAAATGGATGAC ACAGAAACAGGCCTTCTCAGTGCCATCTGTTTAATCTGTGGAGACCGCCAGGACCTTGAGGAACCAACAA AAGTAGACAAGCTCCAAGAACCACTGCTGGAAGCACTAAAGATTTACATTAGAAAACGACGACCCAGCAA GCCTCACATGTTTCCAAAGATCTTAATGAAAATCACAGATCTCCGCAGCATCAGCGCGAAAGGTGCCGAA CGTGTAATTACCTTGAAAATGGAAATTCCTGGATCAATGCCACCTCTCATTCAGGAAATGCTGGAGAATT CTGAAGGACATGAACCCTTGACCCCAAGTTCAAGTGGGAATATAGCAGAGCACAGTCCCAGCGTGTCCCC CAGCTCAGTGGAGAACAGTGGAGTCAGTCAGTCACCACTGCTGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289760
Insert Size	1449 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289760.1, NP_001276689.1
RefSeq Size	3158 bp
RefSeq ORF	1449 bp
Locus ID	218772
UniProt ID	P22605
Gene Summary	Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors (By similarity). The RXRA/RARB heterodimer can act as a repressor on the DR1 element and as an activator on the DR5 element (By similarity). In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (PubMed:19389355).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (beta3) encodes the longest isoform (beta3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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