G3bp2 (NM_001080794) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA409541; E430034L04Rik; G3BP; mKIAA0660
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227927 representing NM_001080794 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTATGGAGAAGCCCAGTCCGCTGCTTGTGGGGCGGGAGTTTGTGAGGCAATATTATACTTTGCTGA ATAAAGCTCCCGAGTATTTGCACAGGTTCTATGGCAGGAATTCCTCCTATGTTCATGGTGGAGTGGATGC CAGTGGAAAGCCCCAGGAAGCAGTTTATGGCCAAAATGATATACACCACAAAGTGTTATCTCTGAACTTC AGTGAATGTCATACCAAAATTCGTCATGTGGATGCTCATGCAACCTTGAGTGATGGTGTAGTGGTACAGG TCATGGGCTTGCTGTCTAACAGTGGACAGCCTGAGAGGAAGTTTATGCAAACCTTTGTTCTGGCTCCAGA AGGATCTGTTCCAAATAAATTTTATGTTCACAATGATATGTTTCGTTATGAAGATGAAGTGTTTGGTGAT TCTGAACCAGAACTTGATGAAGAGTCAGAAGATGAAGTGGAAGAGGAGCAAGAAGACAGGCAGCCGTCTC CTGAACCTGTGCAAGAGAATGCTAACAGCGCTTACTATGACGCACACCCCGTGACTAATGGCATAGAGGA GCCTTTAGAAGAATCCTCTCATGAACCTGAGCCTGAGCCAGAATCTGAAACAAAGACTGAAGAGCTGAAA CCACAAGTGGAGGAGAAACACTTGGAAGAGCTGGAGGAGAAGTCGGCCACTCCTCCTCCTGCTGAGCCTG CTTCTCTGCCTCAGGAACCGCCAAAGGCTTTCTCCTGGGCTTCAGTGACCAGTAAAAACCTGCCTCCTAG TGGTACTGTTTCTTCCTCTGGAATTCCACCCCATGTTAAAGCACCAGTCTCACAGCCGAGGGTTGATGCT AAGCCAGAAGTTCAGTCTCAGCCACCGCGTGTACGGGAACAGCGACCTCGAGAGCGGCCTGGTTTCCCTC CTCGAGGACCAAGGCCAGGCAGAGGAGATATGGAACAGAATGACTCTGACAACCGGAGAATAATTCGCTA TCCGGACAGTCATCAGCTTTTCGTTGGTAACTTGCCTCATGATATTGATGAAAATGAACTGAAAGAATTC TTTATGAGTTTTGGAAACGTTGTGGAACTTCGCATCAATACCAAGGGTGTTGGGGGAAAACTTCCAAATT TTGGTTTTGTGGTTTTTGATGACTCTGAACCAGTTCAGAGAATCTTAATTGCAAAACCAATTATGTTTCG AGGAGAAGTACGTTTAAATGTGGAAGAGAAAAAAACAAGAGCTGCCAGAGAGCGAGAAACCAGAGGTGGT GGCGATGACCGCAGGGATATCAGGCGCAATGATCGGGGTCCTGGTGGTCCACGTGGAATTGTGGGTGGTG GAATGATGCGTGATCGTGACGGAAGAGGGCCACCTCCACGAGGTGGCATGACACAGAAACTTGGTTCTGG AAGAGGAACCGGGCAAATGGAAGGTCGCTTCACAGGACAGCGTCGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001080794
Insert Size	1449 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001080794.2, NP_001074263.1
RefSeq Size	4373 bp
RefSeq ORF	1449 bp
Locus ID	23881
UniProt ID	P97379
Gene Summary	Scaffold protein that plays an essential role in cytoplasmic stress granule formation which acts as a platform for antiviral signaling.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 5, 6, and 7 all encode the same isoform (a).

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