Abi2 (NM_001198571) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	8430425M24Rik; AI839867; C130078H13
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227911 representing NM_001198571 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCTGCAGATGCTGCTGGAAGAGGAAATCCCGGGGGGCCGCCGGGCTCTCTTCGACAGCTACA CGAATCTGGAGCGGGTGGCCGACTACTGCGAGAATAACTACATCCAGTCACCAGATAAGCAGCGAGCCCT AGAAGAAACCAAAGCCTACACCACTCAATCCTTAGCAAGTGTTGCGTATCTGATAAACACCTTGGCCAAC AATGTCCTACAGATGCTGGATATCCAGGCATCACAGTTACGGAGGATGGAGTCTTCAATCAACCATATTT CACAAACAGTTGATATTCATAAAGAGAAGGTTGCAAGAAGAGAAATTGGTATTTTGACCACCAATAAAAA CACTTCAAGGACCCATAAGATTATTGCGCCAGCCAACCTTGAGCGGCCAGTTCGTTATATTCGAAAACCT ATTGACTACACAATTTTAGATGACATTGGACATGGAGTGAAGTGGTTGCTTAGATTTAAGGTGAGTACCC AGAATATGAAAATGGGTGGATTGCCACGTACGACACCTCCAACTCAGAAGCCCCCAAGTCCCCCTATGTC AGGGAAGGGGACACTTGGGCGGCACTCCCCCTATCGAACACTGGAGCCAGTGCGTCCTCCAGTGGTACCA AATGATTACGTACCTAGCCCAACCCGTAATATGGCTCCCTCGCAGCAGAGCCCTGTGAGGACAGCTTCTG TGAATCAAAGAAATCGAACTTACAGCAGCAGTGGGAGCAGTGGAGGAAGTCACCCAAGTAGTCGGAGCAG CAGTCGGGAGAACAGCGGAAGTGGTAGTGTGGGGGTACCCATTGCTGTGCCTACTCCGTCTCCTCCAAGC GTCTTTCCAGACGCTGCTGCTGGGGGTGCACAGAGCCTTGCTGATGGCTTCACTTCTCCAACTCCCCCTG TTATTTCTTCTAACCCCCCCACAGGTCATCCTGTTCAGTTCTACAGCATGAACAGGCCTGCCTCTCGCCA TACACCACCTACAATAGGGGGCTCGTTGCCCTATAGACGTCCTCCTTCCATAACGTCACAAACAAGCCTT CAGAATCAGATGAATGGAGGTCCTTTTTATAACCAGAATCCAGTTTCAGATACACCACCTCCACCCCCAC CTGTGGAAGAGCCAGTCTTTGATGAATCCCCCCCTCCACCGCCTCCTCCAGAAGATTATGAAGAGGAGGA AGCAGCTGTGGTTGAGTACAGCGATCCGTACGCTGAAGAGGACCCACCGTGGGCGCCAAGGGCTTACTTG GAAAAGGTTGTGGCAATTTATGATTATACAAAAGACAAGGAAGATGAGCTGTCCTTTCAGGAAGGAGCCA TTATATATGTCATCAAGAAGAATGACGATGGTTGGTATGAGGGAGTTATGAATGGTGTGACCGGGCTCTT CCCTGGGAACTATGTTGAGTCCATCATGCATTATTCGGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198571
Insert Size	1443 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198571.1, NP_001185500.1
RefSeq Size	5892 bp
RefSeq ORF	1443 bp
Locus ID	329165
UniProt ID	P62484
Gene Summary	Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation (By similarity). Acts as regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge (By similarity). Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (PubMed:15572692). In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (PubMed:27605705). Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (PubMed:15572692). Also required for nascent adherens junction assembly in epithelial cells (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1.

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