Arhgap15 (NM_001301831) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5830480G12Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227899 representing NM_001301831 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAAAGGACAAGCTGCAGTGTGCAGACATCTACAAACTGTGACAACTCTCTGGAAATACTGAATT CTGCCCACCAAGCTACAGGAGCTGTGCAAATGAGAATCAAAAATGCCAACAGCCACCAAGACAGGCAGAG CCAAACTAAATCCATGATCCTCACCGATGCTGGGAAGGTCACTGAACCTATTTCCCGGCACAGAAGGAAT CATTCACAGCATGTGTTGAAAGATGTCATTCCACCACTAGAACATCCAATGGTTGAAAAAGAAGGGTATC TTCAAAAAGCCAAAATTGCAGATGGAGGAAAGAAACTAAGGAAAAACTGGTCTACTTCCTGGATTGTTCT TTCTGGTCGAAAAATTGAATTTTACAAAGACTCCAAGCAGCAAGCTCTTCCTAACATGAAAACCAGGCAC AACGTGGAAAGTGTGGATTTGTGTGGTGCACATATAGAATGGGCCAAAGAAAAATCAAGCAGAAAGAGTG TCTTTCAGATCACAACAGTGTCAGGAAATGAGTTCCTTCTACAGTCAGATATTGACTTCCTCATATTGGA TTGGTTCCAAGCTATCAAAAATGCAATTGACAGATTGCCAAAGAATCCAAGTTGTGGGTCCCTGGAGTTG TTCAATTTGCAGAGATCCTCAAGTTCTGAACTGCCGAGTCACTGCCACATCGATAGAAAAGAACAGAAAC CAGAACACAGGAAGTCCTTCATGTTTCGACTGCACCACAGTGCTTCTGATACAAGTGACAAGAATCGCGT GAAGAGCAGACTGAAGAAGTTCATCTCCAGAAGACCTTCTCTGAAAACTTTGCAGGAAAAGGGACTCATT AAAGATCAAATTTTTGGCTCTCATCTGCACACAGTGTGTGAACGAGAACATTCCACAGTTCCATGGTTTG TAAAGCAATGCATTGAAGCTGTTGAAAAAAGAGGCCTAGACGTTGATGGAATTTATCGAGTTAGTGGCAA TCTTGCAACAATACAGAAGTTAAGATTTATTGTCAACCAAGAAGAGAAGCTGAATTTGGATGACAGCCAG TGGGAGGACATCCACGTCGTCACCGGAGCACTGAAGATGTTTTTCCGGGAGCTGTCTGAACCGCTCTTCC CTTACAGTTTCTTTGAGCGGTTTGTGGAGGCGATCAAAAAACAAGACAGCAATGAAAAAATTGAAACTAT GAGGTCTCTGGTAAAACGTCTCCCTCCACCAAATCATGATACCATGAAAATCCTCTTCAGACATCTAACC AAATTCATAATCTTCAAACGCACCAGCTTCTTGATTTCCAATCAGATGGGATTTAGCCTGATCCAGACCC CAGCTGTGTCCTGTTCCTGCCTACTAAGGGCACTGCATCAGAGAACATGTTTTCTCTCTAGAGGGAGACC TTTCCAAGACCCTGCCTCCCAACTCTCCCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301831
Insert Size	1434 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301831.1, NP_001288760.1
RefSeq Size	3118 bp
RefSeq ORF	1434 bp
Locus ID	76117
Gene Summary	The protein encoded by this gene is a RAC GTPase-activating protein that is regulated through its PH domain and by recruitment to the membrane. The protein accelerates hydrolysis of guanosine triphosphate to guanosine diphosphate to repress Rac activity. Knock-out of Arhgap15 function demonstrates that this gene is required to regulate multiple functions in macrophages and neutrophils. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014] Transcript Variant: This variant (3) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1.

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