Trpc7 (NM_001302373) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	TR; TRP7; Trrp8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227893 representing NM_001302373 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTTGTGGCCCATGCAGTGTCTTTTACCATCTTCTTGGGACTGCTAGTTGTGAACGCCTCCGACC GGTTTGAGGGCGTTAAGACTCTGCCCAATGAGACCTTCACAGACTACCCCAAACAGATCTTCAGAGTGAA AACCACACAGTTCTCCTGGACGGAGATGCTCATCATGAAGTGGGTCTTAGGAATGATCTGGTCCGAATGC AAGGAGATCTGGGAGGAGGGGCCGCGGGAGTACGTGCTACACTTGTGGAACCTTCTGGATTTTGGCATGC TGTCCATCTTCGTGGCCTCCTTCACTGCGAGGTTCATGGCTTTCCTCAAGGCCAGCGAGGCCCAGCTGTA CGTGGACCAGTACGTGCAGGATGTAACGCTGCACAACGTCTCACTTCCACCGGAAGTGGCCTACTTCACC TACGCCAGGGATAAGTGGTGGCCTTCAGACCCCCAGATCATCTCGGAAGGGCTGTACGCCATTGCGGTTG TCCTCAGCTTCTCCCGGATCGCCTACATCCTGCCAGCCAATGAGAGCTTCGGACCTTTGCAGATCTCCCT GGGAAGAACTGTGAAAGACATCTTCAAGTTCATGGTCATTTTTATCATGGTATTTGTGGCCTTTATGATC GGAATGTTCAACCTGTACTCCTACTACCGAGGTGCAAAGTACAACCCAGCGTTTACCACAGTTGAAGAAA GCTTTAAAACCTTGTTTTGGTCCATATTTGGCTTGTCTGAGGTCATCTCGGTGGTGCTGAAATACGACCA CAAGTTCATCGAGAATATTGGCTACGTGCTGTATGGGGTTTATAATGTCACCATGGTGGTTGTACTACTC AACATGCTGATAGCCATGATCAACAACTCCTATCAGGAAATCGAGGAAGACGCGGACGTGGAGTGGAAAT TTGCTCGAGCGAAGCTCTGGCTTTCTTACTTTGATGAAGGAAGAACTCTCCCTGCTCCCTTTAACCTGGT GCCGAGCCCTAAATCCTTTTATTATCTCATAATGAGAATCAAGATGTGCCTCATAGAGCTCTGCCAATCT AAGGCCAAACGCTGTGAAAACGACCTGGAAATGGGCATGCTGAACTCCAAGTTCAGGAAGACTCGCTACC AGGCTGGCATGAGGAATTCTGAAAACCTGACAGCCAATAGCACCTTCAGCAAGCCCACCAGATACCAGAA GATCATGAAGCGGCTCATAAAGAGATACGTGCTGAAGGCCCAGGTGGACAGAGAGAACGATGAAGTCAAT GAAGGTGAACTCAAGGAGATCAAACAAGACATCTCAAGCCTTCGCTATGAGCTCCTGGAGGAGAAGTCTC AGGCTACAGGAGAGCTGGCAGACCTGATCCAGCAGCTCAGCGAGAAGTTTGGGAAGAATCTGAACAAAGA CCACCTGCGGGTGAACCAGGGCAAGGACATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302373
Insert Size	1434 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302373.1, NP_001289302.1
RefSeq Size	3674 bp
RefSeq ORF	1434 bp
Locus ID	26946
Gene Summary	The protein encoded by this gene is a member of the transient receptor potential channel family of proteins, which form six-transmembrane cation-permeable channels that are calcium permeant. Knock out mice are viable but display a reduction in the gamma wave activity that precedes seizure induction in response to a muscrarinic agonist, suggesting a functional role for this protein in initiation of seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (3) differs in the 5' UTR and uses a downstream start codon compared to variant 1. It encodes isoform 3, which has a shorter N-terminus compared to isoform 1.

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