Hadhb (NM_001289798) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930479F15Rik; Mtpb; TP-beta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227884 representing NM_001289798 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTACCATCTTGACTTCCACTTTTAGAAACTTGTCAACTACATCAAAATGGGCTCTCAGATCTTCTA TAAGACCTCTGAGCTGTTCTTCCCAACTGCACTCTGCCCCAGCTGTCCAGACCAAGTCAAAGAAGACTTT AGCAAAACCCAATATGAAGAATATTGTGGTGGTGGAAGGGGTCCGCATTCCATTTCTGCTGTCAGGCACT TCGTATAAAGACCTAATGCCACATGACTTGGCTAGAGCTGCACTTTCGGGTTTGTTGCATCGGACCAATA TTCCAAAGGATGTTGTTGATTATATCATCTTTGGTACAGTTATTCAGGAAGTGAAAACAAGCAATGTGGC TAGAGAGGCTGCCCTGGGAGCTGGCTTCTCTGATAAGACTCCAGCTCACACTGTCACCATGGCTTGTATC TCTTCAAACCAAGCCATGACCACAGCTGTTGGTCTGATAGCTTCTGGCCAGTGTGATGTCGTCGTGGCTG GTGGTGTTGAGTTAATGTCTGATGTCCCTATTCGTCATTCAAGAAATATGAGGAAAATGATGCTTGATCT CAATAAAGCCAAGACTCTGGGCCAGCGCCTGTCCTTACTCAGTAAATTCAGATTGAATTTTCTGTCCCCT GAGCTCCCTGCAGTGGCTGAGTTCTCCACTAATGAGACCATGGGCCACTCTGCAGACCGACTGGCTGCTG CCTTTGCTGTTTCTCGAATGGAACAGGATGAATATGCACTGCGTTCTCATAGTCTGGCCAAGAAGGCACA GGATGAAGGACACCTTTCTGATATTGTACCCTTCAAAGTACCAGGAAAGGACACAGTTACCAAAGATAAT GGGATCCGTCCTTCCTCACTGGAGCAAATGGCCAAACTAAAACCTGCGTTCATCAAACCCTATGGCACAG TGACAGCTGCAAATTCTTCTTTCCTGACTGATGGCGCTTCTGCGATGCTAATCATGTCAGAGGACAGAGC TCTGGCCATGGGTTATAAACCAAAGGCATATTTGAGGGATTTTATATATGTGTCCCAGGATCCGAAAGAT CAGCTTTTACTCGGACCAACATATGCTACTCCAAAAGTTTTAGAAAAGGCAGGGTTAACCATGAATGATA TCGATGCTTTTGAATTTCATGAAGCCTTCTCAGGCCAGATTTTAGCTAACTTTAAAGCCATGGATTCTGA TTGGTTTGCACAAAACTACATGGGTAGGAAAACCAAGGTTGGATCACCTCCTCTGGAGAAGTTTAATATC TGGGGCGGATCACTGTCTCTGGGGCACCCTTTTGGAGCCACTGGCTGTCGGCTGGTCATGGCAGCTGCCA ACAGACTGAGGAAGGATGGAGGCCAGTATGCTTTAGTGGCTGCCTGTGCAGCTGGAGGACAGGGTCATGC TATGATTGTGGAAGCTTACCCCAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289798
Insert Size	1428 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289798.1, NP_001276727.1
RefSeq Size	2092 bp
RefSeq ORF	1428 bp
Locus ID	231086
UniProt ID	Q99JY0
Gene Summary	Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.