Bpifb1 (NM_153418) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Lplunc1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227875 representing NM_153418 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGCCCGTGGATTATCACCCTCCTCTGTGGTTTGCTGGGAGCCACACTGGTCCAAGCTAACGTCT ATCCCCCTGCAGTGCTCAACCTTGGCCCAGAAGTCATCCAGAAACACCTGACCCAGGCACTGAAGGACCA TGATGCCACTGCCATCCTCCAGGAGTTGCCACTGCTCAGAGCCATGCAAGATAAGTCTGGCAGTATCCCC ATACTGGACAGCTTCGTGCACACCGTTCTGAGATACATCATATGGATGAAGGTCACCTCTGCTAACATCC TCCAGCTGGATGTGCAGCCTTCAACTTATGACCAGGAGCTGGTGGTCAGAATCCCCCTGGACATGGTGGC TGGACTAAACACACCACTGATCAAGACCATAGTGGAGTTCCAAATGAGCACCGAGGTCCAAGCCCTCATC CGGGTGGAGAGGAGCAAGAGCGGCCCCGCCCACCTGAATCTCAGCGACTGCTCCAGCAATGAGAGCACCC TGCGCCTCAGCCTGCTTCACAAGCTCTCCTTCGTGGTCAACTCCTTGGCAAAGAATGTTATGAATCTCCT GGTGCCAGCCCTGCCCCAAATAGTGAAAAACCACCTGTGCCCTGTGATCCAGCAGGCCTTTGATGACATG TACGAAGACTTCCTGAGACTGACAACAGCACCCATTGCCCTCAGTCCTGGAGCCCTGGAGTTTGGCCTTC TGTCTCCTGCTATCCAGGACAGTAATATCCTGTTGAACCTGAAGGCCAAGCTGCTGGACTCACAGGCGAG GGTAACCAACTGGTTCAACAACTCTGCGACTTCTCTGATGGAGACCACCCCAGACAGGGCCCCCTTCAGC CTGACCGTGAGGCAGGACCTGGTGAATGCCATTGTGACCACCCTGGTCCCCAAGGAGGAGCTTGTAATCC TGCTCAGATTCGTGATTCCTGATGTGGCCCGCCAGTTACAGATGGACATCAAGGAAATCAATGCAGAGGC AGCCAACAAGCTGGGGCCCACCCAGATGTTGAAGATCTTCACCCACAGCACCCCCCACATTGTGCTGAAC GAGGGCAGTGCCAGGGCAGCCCAAAGTGTCGTGCTGGAAGTGTTCCCTACCAACACTGATGTCCGGCCCT TCTTCTCTCTCGGCATTGAGGCCAGTTATGAAGCTCAGTTCTTCACAGAAGACAACCGGCTTATGCTCAA CTTCAATAACGTCAGTATTGAGCGCATCAAGCTGATGATCTCAGATATCAAACTATTCGATCCTGAAGTC CTGAAGGACACCCTGACCAAGATCCTTGAATACACACTGCTGCCCAATGAGAATGGCAAACTGAGAACTG GAGTCCCCATGTCAATGTCCAAGGCCTTGGGATACGAGAAAGCCATGTGGTCTGTGAGCAAGGGTGCCCT CAAGCTCACTCCAGCCTCCTCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_153418
Insert Size	1425 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_153418.2, NP_700467.2
RefSeq Size	1653 bp
RefSeq ORF	1425 bp
Locus ID	228801
UniProt ID	Q61114
Gene Summary	May play a role in innate immunity in mouth, nose and lungs. Binds bacterial lipopolysaccharide (LPS) and modulates the cellular responses to LPS. May be involved in formation of the left-right axis in the node of the developing embryo.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate 5' exon, as compared to variant 1. Both variants 1 and 2 encode the same protein.

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