Nfatc2 (NM_001291176) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI607462; NF-ATc2; NF-ATp; NFAT1; NFAT1-D; Nfatp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227873 representing NM_001291176 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACAAGCCTCTGGGGCTTCAGATCTTCATTGGGACAGCAGATGAGAGGATCCTTAAGCCGCACG CCTTCTACCAAGTACACAGGATCACTGGGAAAACGGTCACCACCACGAGCTATGAGAAGATCGTAGGCAA CACCAAGGTCCTGGAGATCCCCCTGGAGCCAAAGAACAACATGAGAGCCACCATCGACTGTGCAGGCATC CTGAAGCTCCGAAACGCTGACATCGAGCTGCGGAAGGGCGAGACGGACATCGGCAGGAAGAACACGCGTG TGCGCCTGGTGTTCCGCGTGCACGTCCCAGAGCCCAGTGGGCGCATCGTCTCCCTGCAGGCTGCGTCCAA CCCCATCGAGTGCTCTCAGCGCTCTGCCCACGAGCTGCCCATGGTGGAGAGACAAGACATGGACAGCTGC CTGGTCTACGGGGGCCAGCAGATGATCCTCACGGGCCAGAACTTCACAGCGGAGTCCAAGGTTGTGTTCA TGGAGAAGACTACAGATGGGCAGCAGATTTGGGAGATGGAAGCTACGGTGGATAAAGACAAGAGCCAGCC TAACATGCTTTTTGTTGAGATCCCCGAGTATCGGAACAAGCACATCCGCGTGCCCGTGAAAGTCAACTTC TACGTCATCAACGGAAAGAGGAAACGAAGTCAGCCACAGCACTTTACCTACCACCCAGTCCCTGCCATCA AGACAGAGCCCAGCGATGAGTATGAACCATCTTTGATCTGCAGCCCCGCCCATGGAGGCCTGGGGAGCCA GCCATATTACCCACAGCACCCAATGCTGGCCGAGTCCCCCTCCTGCCTTGTGGCTACCATGGCCCCCTGC CAACAGTTCCGCTCGGGGCTCTCATCCCCCGATGCTCGCTACCAACAGCAGAGCCCCGCAGCTGCCCTCT ACCAGAGAAGCAAGAGCCTGAGTCCCGGCCTGCTGGGCTACCAGCAGCCGTCCCTCCTGGCAGCACCCTT GGGTCTGGCTGATGCCCACCGCTCTGTGCTGGTGCATGCTGGTTCTCAGGGGCAGGGGCAGGGCTCCACC CTGCCACACACATCCTCGGCCAGCCAGCAGGCCTCACCCGTGATCCACTACTCACCCACCAACCAGCAGC TTCGCGGTGGGGGTCACCAGGAGTTCCAGCATATCATGTACTGTGAAAACTTCGGCCCCAGCTCTGCCAG GCCTGGCCCGCCTCCCATCAACCAAGGTCAGAGGCTGAGCCCGGGCGCCTACCCCACAGTCATCCAACAA CAGACTGCCCCGAGCCAAAGAGCTGCCAAAAACGGACCCAGTGACCAGAAGGAAGCTCTGCCCACGGGAG TGACCGTCAAACAGGAACAGAACCTGGACCAGACCTACCTGGATGACGAGTTGATAGACACACACCTTAG CTGGATACAAAACATATTATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001291176
Insert Size	1422 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291176.1, NP_001278105.1
RefSeq Size	5809 bp
RefSeq ORF	1422 bp
Locus ID	18019
Gene Summary	Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (13, also known as IB-deltaII-Xa) contains alternate 5' and 3' exon structure, and it thus differs in both UTRs, initiates translation at a downstream in-frame start codon, and differs in the 3' coding region, compared to variant 1. The encoded isoform (h) is shorter at at the N-terminus and has a distinct C-terminus, compared to isoform a. Both variants 8 and 13 encode isoform h.

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