Slc17a5 (NM_001276452) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4631416G20Rik; 4732491M05; AST; ISSD; NSD; SD; SIALIN; SIASD; SLD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227857 representing NM_001276452 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCCCCTGCTTCGGGGTCCGGCGGGAAACGACGATGAGGAGAGCTCGGACAGCACCCCGCTCCTGC CGGGCGCCCGGCAGACCGAAGCGGCTCCAGTGTGCTGCTCTGCTCGGTACAACTTAGCGATTTTGGCGTT CTGTGGTTTCTTCGTTCTCTATGCCTTACGGGTGAACCTGAGTGTTGCGTTAGTGGACATGGTAGATTCA AATACAACTCTGACTGATAATAGAACGTCTAAGGAGTGTGCGGAACATTCTGCCCCCATAAAAGTTCACC ACAATCACACAATTCCCGGTGGGTACATTGCCAGCAGGGTCGGAGGGAAGCTGCTGCTGGGCCTGGGCAT CTTAGGCACCTCCGTCTTCACCCTGTTCACACCGCTGGCCGCAGACTTAGGCGTGGTGACTCTCGTTGTG CTTAGAGCGCTGGAAGGACTGGGAGAGGGTGTTACGTTTCCAGCTATGCACGCCATGTGGTCTTCCTGGG CTCCCCCTCTGGAAAGAAGCAAGCTTCTTACCATTTCCTATGCGGGAGCACAGCTTGGGACAGTGATCTC ACTTCCTCTTTCCGGAATAATATGCTACTATATGAACTGGACTTACGTCTTCTATCTTTTTGGTATAGTT GGAATTGTCTGGTTTATTTTATGGATGTGGATAGTCAGTGATACACCAGAAACTCACAAGACAATCTCCC ATTATGAAAAAGAATACATTGTTTCATCATTAAAAAATCAGCTTTCTTCGCAGAAGGTGGTGCCGTGGGG GTCCATCTTGAAGTCACTGCCACTTTGGGCAATTGTGGTAGCACATTTCTCCTACAACTGGTCTTTTTAC ACCTTATTGACGCTACTGCCAACTTATATGAAGGAAATCCTAAGGTTCAATGTTCAAGAGAACGGGTTTT TATCTGCATTGCCTTATTTTGGCTGTTGGTTATGCATGATCCTCTGTGGTCAAGCCGCTGACTATTTAAG GGTCAAGTGGAACTTTTCAACTATAAGCGTTCGGAGAATTTTTAGCCTCGTAGGAATGGTTGGCCCTGCG GTTTTCCTAGTCGCGGCTGGATTTATAGGCTGTGACTATTCCTTGGCCGTTGCGTTCCTAACCATATCCA CGACGCTGGGAGGCTTCGCCTCTTCTGGATTTAGCATCAACCATCTGGATATCGCTCCTTCGTATGCTGG CATCCTCTTGGGCATCACAAACACGTTTGCCACTATTCCCGGGATGACGGGACCCATCATTGCTAAAAGT CTGACCCCTGATAACACTATTAGGGAATGGCAGACTGTCTTCTGTATCGCTGCTGCTATTAACGTGTTTG GGGCCATTTTCTTCACGCTGTTTGCCAAAGGGGAAGTGCAGAGCTGGGCTCTCAGTGACCACCACGGACA CAGAAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276452
Insert Size	1410 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276452.1, NP_001263381.1
RefSeq Size	3163 bp
RefSeq ORF	1410 bp
Locus ID	235504
UniProt ID	Q8BN82
Gene Summary	Transports glucuronic acid and free sialic acid out of the lysosome after it is cleaved from sialoglycoconjugates undergoing degradation, this is required for normal CNS myelination. Mediates aspartate and glutamate membrane potential-dependent uptake into synaptic vesicles and synaptic-like microvesicles. Also functions as an electrogenic 2NO(3)(-)/H(+) cotransporter in the plasma membrane of salivary gland acinar cells, mediating the physiological nitrate efflux, 25% of the circulating nitrate ions is typically removed and secreted in saliva (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform b which is shorter than isoform a.

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