Smad2 (NM_001252481) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	7120426M23Rik; Madh2; Madr2; mMad2; Smad-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227845 representing NM_001252481 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCATCTTGCCATTCACTCCGCCAGTGGTGAAGAGACTTCTGGGATGGAAAAAATCAGCCGGTG GGTCTGGAGGAGCAGGTGGTGGAGAGCAGAATGGACAGGAAGAAAAGTGGTGTGAAAAAGCAGTGAAAAG TCTGGTGAAAAAGCTAAAGAAAACAGGACGGTTAGATGAGCTTGAGAAAGCCATCACCACTCAGAATTGC AATACTAAATGTGTCACCATACCAAGCACTTGCTCTGAAATTTGGGGACTGAGTACAGCAAATACGGTAG ATCAGTGGGACACAACAGGCCTTTACAGCTTCTCTGAACAAACCAGGTCTCTTGATGGCCGTCTTCAGGT TTCACACCGGAAAGGGTTGCCACATGTTATATATTGCCGGCTCTGGCGCTGGCCGGACCTTCACAGTCAT CATGAGCTCAAGGCAATCGAAAACTGCGAATATGCTTTTAATCTGAAAAAAGATGAAGTGTGTGTAAATC CGTACCACTACCAGAGAGTTGAGACCCCAGTCTTGCCTCCAGTCTTAGTGCCTCGGCACACGGAGATTCT AACAGAACTGCCGCCCCTGGATGACTACACCCACTCCATTCCAGAAAACACAAATTTCCCAGCAGGAATT GAGCCACAGAGTAATTACATCCCAGAAACACCACCACCTGGATATATCAGTGAAGATGGAGAAACAAGTG ACCAACAGTTGAACCAAAGTATGGACACAGGCTCTCCGGCTGAACTGTCTCCTACTACTCTCTCTCCTGT TAATCACAGCTTGGATTTGCAGCCAGTTACTTACTCGGAACCTGCATTCTGGTGTTCAATCGCATACTAT GAACTAAACCAGAGGGTTGGAGAGACCTTCCATGCGTCACAGCCCTCGCTCACTGTAGACGGCTTCACAG ACCCATCAAACTCGGAGAGGTTCTGCTTAGGCTTGCTCTCCAACGTTAACCGAAATGCCACTGTAGAAAT GACAAGAAGACATATAGGAAGGGGAGTGCGCTTGTATTACATAGGTGGGGAAGTGTTTGCTGAGTGCCTA AGTGATAGTGCAATCTTTGTGCAGAGCCCCAACTGTAACCAGAGATACGGCTGGCACCCTGCAACAGTGT GTAAGATCCCACCAGGCTGTAACCTGAAGATCTTCAACAACCAAGAATTTGCTGCTCTTCTGGCTCAGTC TGTCAACCAGGGTTTTGAAGCCGTTTATCAGCTAACCCGAATGTGCACCATAAGAATGAGTTTTGTGAAG GGCTGGGGAGCAGAATATCGGAGGCAGACAGTAACAAGTACTCCTTGCTGGATTGAACTTCATCTGAATG GCCCTCTGCAGTGGCTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGTGCGATGCTCAAGCATGTC GTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252481
Insert Size	1404 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252481.1, NP_001239410.1
RefSeq Size	8863 bp
RefSeq ORF	1404 bp
Locus ID	17126
UniProt ID	Q62432
Gene Summary	Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.