Adra1a (NM_001271760) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Adr; Adra1c
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227844 representing NM_001271760 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTTCTTTCTGAAAATGCTTCTGAAGGCTCCAACTGCACCCACCCGCCAGCACAGGTGAACATTT CTAAGGCCATTCTACTTGGGGTGATCTTGGGGGGCCTCATCATTTTCGGGGTCTTGGGGAATATTTTAGT GATCCTCTCGGTGGCCTGTCATCGGCATCTGCACTCGGTGACTCACTACTACATTGTCAACCTGGCTGTG GCAGACCTCCTCCTCACCTCCACCGTGCTGCCCTTCTCTGCCATCTTTGAGATCCTGGGCTACTGGGCCT TTGGCAGGGTGTTCTGCAACATCTGGGCGGCGGTGGACGTCTTATGCTGCACAGCGTCCATCATGGGCCT CTGCATCATCTCCATCGACCGATACATTGGTGTGAGCTACCCGCTGCGCTACCCCACCATTGTCACCCAG AGGAGGGGCGTCAGGGCTCTGCTCTGCGTCTGGGCGCTTTCCTTGGTCATCTCCATCGGACCCCTGTTCG GCTGGAGGCAGCAGGCTCCGGAGGATGAGACCATCTGCCAAATCAATGAGGAGCCAGGATACGTGCTGTT CTCAGCGCTGGGCTCTTTCTACGTGCCACTGACCATCATCCTGGTTATGTACTGTCGAGTCTACGTGGTA GCCAAGAGAGAAAGCCGAGGCCTCAAGTCCGGCCTCAAGACCGACAAGTCAGACTCAGAGCAAGTGACGC TCCGTATCCACCGTAAAAATGTCCCTGCAGAAGGCAGCGGAGTAAGCAGTGCCAAGAATAAGACTCACTT CTCCGTGAGGCTGCTCAAGTTTTCCCGAGAGAAGAAAGCCGCCAAGACGCTGGGCATTGTGGTGGGATGC TTCGTCCTCTGCTGGCTGCCATTCTTCCTCGTGATGCCCATTGGGTCCTTCTTCCCGAATTTCAAGCCAC CGGAAACAGTTTTCAAAATAGTATTTTGGCTTGGGTACCTAAATAGTTGCATCAACCCTATCATATACCC ATGCTCCAGCCAGGAGTTCAAGAAAGCCTTTCAGAATGTGCTGCGAATCCAGTGTCTTCGCAGAAGGCAG TCTTCCAAGCATGCCCTGGGCTACACTCTGCACCCACCCAGCCAGGCTGTAGAGGGGCAGCACAGAGGCA TGGTGCGTATCCCGGTGGGCTCAGGAGAGACTTTCTATAAGATCTCCAAGACAGATGGAGTCCGTGAATG GAAGTTTTTCTCTTCCATGCCCCAGGGATCGGCCAGGATTACCATGCCGAAGGACCAATCCGCCTGTACC ACAGCCCGGGTGAGAAGTAAAAGCTTTTTGCAGGTCTGCTGCTGTGTGGGGTCGTCGACCCCACGCCCTG AAGAAAATCACCAAGTTCCAACCATTAAGATCCACACCATCTCCCTCGGTGAAAACGGGGAGGAAGTCTA G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271760
Insert Size	1401 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271760.1, NP_001258689.1
RefSeq Size	3814 bp
RefSeq ORF	1401 bp
Locus ID	11549
UniProt ID	P97718
Gene Summary	This gene encodes one of several multipass transmembrane proteins that function as G protein-coupled receptors. The encoded protein binds to epinephrine and norepinephrine to mediate signaling in cells of the cardiac, nervous, and other organ systems. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Both variants 1 and 3 encode the same isoform (1).

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