Slc30a6 (NM_001252478) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9530029F08Rik; ZnT6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227836 representing NM_001252478 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACGATTCATCTCTTTCGAAAGCCACAGAGATCTTTTTTTGGAAAGTTACTACAGGAATTTAGAC TTGTCGCAGCTGACCGGAGGTCCTGGAAGATCCTTCTGTTCGGTGCAATCAACGTGCTGTGCACTGGCTT CCTGCTCATGTGGTGCAGCTCCACCAACAGCATCGCATTAACTGCCTATACTTACCTCACCATCTTTGAT CTCTTTAGTTTGTTTTTCTTTAGTTTAATAACATGTTTAATAAGCTACTGGGTAATGATGAGGAAACCTA GCCCTGTCTATTCATTTGGGTTTGAAAGATTAGAAGTCCTGGCTGTATTTGCTTCTACTGTCTTGGCACA ATTGGGAGCCCTCTTTATACTAAAAGAAAGTGCAGAACGCTTTTTGGAGCAGCCCGAGATACACACGGGA AGATTGTTAGTTGGTACTTTTGTGGCCCTTTCTTTCAACCTGTTCACGATGCTTTCTATTCGGAATAAGC CTTTTGCTTACGTCTCTGAAGCTGCTAGTACGAGCTGGCTTCAGGAGCACGTGGCAGACCTTAGCCGCAG CTTGTGTGGCCTTATCCCAGGACTCAGCAGTATCTTCCTGCCCCGCATGAATCCGTTTGTTCTGATTGAT CTTGCTGGGGCGTTTGCTCTGTGTATAACATACATGCTGATTGAGATTAATAACTACTTTGCCGTGGACA CTGCGTCAGCCATTGCCATTGCCCTGATGACGTTTGGCACCATGTACCCCATGAGTGTGTACAGTGGGAA AGTGCTCCTCCAGACAACACCACCTCATGTTATCGGTCAGTTGGACAAACTGATTAGAGAGGTGTCTACC TTGGATGGGGTGCTAGAAGTCCGAAATGAACACTTTTGGACCCTTGGATTTGGCTCATTGGCTGGGTCAG TGCATGTACGGATCCGACGAGATGCGAACGAACAAATGGTCCTTGCTCATGTGAGCAACAGGCTGTGCAC GCTGGTGTCCACGCTGACTGTTCAGATCTTCAAGGACGACTGGATTCGGCCTGCCTTATCCTCTGGGCCT GTTGCGCCCAACGTCCTAAACTTCTCAGACCATCACGTAATCCCAATGCCTTTATTAAAGAACGTGGATG AGAGGACCCCCGTCACGTCAACCCCAGCCAAGCCCAGCAGCCCACCTCCGGAGTTTTCATTTAATACGCC TGGGAAAAACGTGAGCCCGGTTATTCTTCTGAACACACAGACAAGGCCATATAGTTTGGGTCTTAATCGC GGACACACACCATACAGCAGCGTGTTCAGCCAAGGACTCGCATTTCCAGGAGTTGGAGCAGGCCAGGGAT TGAGGCCCACCTTCCCACATATACCAAGTAGATATGGAATCAACAGGATGGGGCAGCCAAGACCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252478
Insert Size	1398 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252478.1, NP_001239407.1
RefSeq Size	2171 bp
RefSeq ORF	1398 bp
Locus ID	210148
Gene Summary	Zinc-efflux transporter which allocates the cytoplasmic zinc to the trans-Golgi network (TGN) as well as the vesicular compartment.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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