Tcf12 (NM_001253865) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A130037E08Rik; ALF1; bHLHb20; HEB; HEBAlt; HTF-4; HTF4; ME1; REB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227827 representing NM_001253865 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCGCTAGCACTTTCTTTATGCAAGATGGGACCCACAGTTCTTCTGACCTTTGGAGTTCATCGAATG GGATGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCACATCCCACATGTCTCAGTCCAGTAGTTA TGGCAGCCTTCATTCACATGACCGCTTGAGTTATCCTCCACACTCAGTGTCACCAACAGACATAAACACA AGTCTTCCGCCAATGTCCAGCTTCCACCGTGGTAGTACCAGCAGCTCACCATATGTTGCCGCCTCACATA CTCCTCCCATCAATGGATCAGATAGCATCCTAGGAACCAGAGGGAATGCTGCTGGAAGCTCACAGACGGG TGATGCACTTGGGAAGGCCTTGGCATCTATTTATTCCCCTGACCACACAAGCAGTAGTTTTCCATCAAAC CCATCAACACCAGTGGGATCTCCTTCACCTCTCACAGGTACCAGTCAGTGGCCCAGAGCTGGAGGGCAAG CTCCTTCATCTCCAAGCTATGAAAACTCACTTCACTCCCTGCAGTCTCGAATGGAAGACCGCTTAGACAG GCTGGATGATGCTATCCATGTGCTACGAAACCATGCAGTTGGACCTTCTACCAGTCTGCCTACTAGCCAC AGTGACATACACAGTTTGCTGGGACCATCCCATAATGCATCAATTGGAAACCTCAATTCAAACTATGGAG GATCCAGCCTTGTTACAAATAGTCGATCAGCTTCGATGGTCGGAACACATCGGGAAGATTCAGTCAGTCT CAATGGCAATCATTCGGTCCTGTCTAGTACTGTTGCTGCCTCAAACACAGAACTGAACCATAAAACACCA GAAAATTTCAGAGGTGGTGTACAAAATCAGTCTGGAAGTGTTGTTCCAACAGAAATCAAGACTGAAAACA AAGAAAAAGATGAAAACCTTCATGAACCTCCTTCATCAGATGACATGAAATCAGATGATGAGTCCTCCCA GAAAGACATCAAGGTCTCATCTAGGGGCAGAACAAGCAGTACCAATGAAGACGAGGATCTGAATCCAGAA CAGAAAATCGAAAGGGAGAAGGAAAGGCGGATGGCTAACAATGCCAGAGAGCGCCTGCGCGTGCGGGATA TTAACGAGGCGTTCAAGGAGCTTGGCCGAATGTGTCAGCTTCATTTGAAGAGTGAAAAACCTCAGACAAA ACTTCTCATTCTTCATCAGGCCGTGGCAGTCATCCTTAGTCTAGAACAGCAAGTGAGAGAGAGGAACCTC AACCCCAAAGCAGCCTGCCTTAAGAGAAGAGAAGAAGAAAAAGTCTCTGCTGCGTCAGCAGAGCCGCCCA ACACGTTGCCAGGAGCCCATCCTGGGCTTAGTGAGTCTACCAACCCTATGGGTCATCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253865
Insert Size	1392 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253865.1, NP_001240794.1
RefSeq Size	4204 bp
RefSeq ORF	1392 bp
Locus ID	21406
UniProt ID	Q61286
Gene Summary	Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) lacks several exons in the coding region, compared to variant 1. The resulting protein (isoform 5) is shorter when it is compared to isoform 1.

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