Gopc (NM_001199272) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2210402P09Rik; AI844555; CAL; FIG; GOPC1; PIST
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227826 representing NM_001199272 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGGGTGGCCCATGCCCGGCGGGAGCCGGCGGCGGCCCAGGGGGCTCCTCCTGCCCTGTGGGGG TCTCCCCTGGCGGGGTGTCCATGTTCCGATGGCTTGAGGTGCTGGAGAAGGAGTTCGACAAGGCCTTCGT GGATGTGGATCTGCTTTTGGGCGAGATCGATCCGGACCAGGCGGACATCACTTATGAGGGGCGTCAGAAG ATGACCAGCCTTAGCTCCTGCTTTGCGCAGCTATGCCACAAAGCCCAGACTGTGTCCCAGATCAACCACA AGCTTGAGGCACAGTTGGTGGATCTGCGATCGGAATTGACAGAGACTCAAGCAGAGAAAGTTGTTCTGGA GAAAGAAGTCCACGAGCAGCTTTTACAGCTGCACTCCACTCAGCTTCAGCTTCATGCCAAGACTGGTCAA AGTGTTGACTCTGGGGCCATCAAGGCAAAATTGTCTGTCCACTCTGTGGAGGATCTGGAAAGAGAACTTG AAGCAAATAAAACAGAAAAGGTAAAAGAAGCTCGACTTGAAGCTGAAGTGAAGCTACTAAGGAAGGAGAA CGAGGCTCTTCGAAGGCACATAGCTGTTCTCCAGGCTGAAGTTTATGGGGCGAGGTTGGCTGCCAAGTAC TTGGATAAGGAGCTGGCCGGCAGGGTGCAACAGATACAATTACTGGGACGGGATATGAAGGGACCTGCTC ACGATAAGCTCTGGAACCAGTTAGAAGCTGAAATACATTTGCATCGCCACAAAACTGTCATCAGAGCCTG CAGAGGGCGCAACGACTTGAAGCGGCCCATGCAAGCACCCCCAGGCCACGATCAAGACTCTTTGAAGAAA AGCCAGGGAGTCGGTCCAATTAGAAAAGTTCTCCTCCTTAAGGAAGACCATGAAGGCCTGGGCATTTCTA TCACAGGTGGGAAAGAGCACGGTGTTCCCATCCTCATCTCTGAGATCCACCCGGGACAGCCTGCGGATAG ATGTGGAGGGCTGCATGTTGGAGATGCTATTCTGGCAGTCAATGGAGTCAACCTAAGAGACACAAAGCAT AAAGAAGCTGTAACTATACTCTCCCAGCAGAGAGGAGAGATTGAGTTTGAAGTAGTCTACGTGGCACCTG AAGTGGATTCTGACGATGAAAATGTAGAGTATGAAGACGAGAGTGGACACCGCTATCGTCTGTATCTTGA CGAGTTAGAAGGCAGTGGGAATTCTGGTGCTAGTTGCAAAGACAGCAGTGGCGAGATGAAAATGTTACAA GGGTATAACAAGAAGGCAGTGAGGGATGCGCATGAGAATGGGGACGTGGGGGCTGCAGGTGAGAGCCCTC TGGATGACACTGCTGCGAGAGCGGCTCATCTGCACAGCCTGCATCAGAAGAAGGCCTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199272
Insert Size	1392 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199272.1, NP_001186201.1
RefSeq Size	4319 bp
RefSeq ORF	1392 bp
Locus ID	94221
UniProt ID	Q8BH60
Gene Summary	Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) is the longer transcript and it encodes the longer protein (isoform a).

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