Shroom2 (NM_001290685) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4832440C16; Apxl; C630003H05Rik; Shrm2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227820 representing NM_001290685 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGACACTTTCCATCCTCTGCTATCTTTCTATTTGGCTGGTTGACAGTGGGTCTTTGTGTTGGGATC CCTGTGTGGGTCGTGGGTGGAAATCTGTACATGTCAAGATTTCAGAGGACAGCCCTTATCTTTGCAGAAT CGAGCGGGTGATGGACAACAACACCACTGTGAAGATGGTGCCCATAAAAATTGTGCACTCAGAAAGCCAG CCCGAGAAGGAGAGTCGTCAGAGTCTCGCGTGCCCAGCTGAGCTGCCCCCACTGCCCAGTGGGCTGGAGA GGGACCAGATCAAGACACTGAGTACATCAGAGCAATGCTACTCCCGCTTCTGTGTGTACACACGACAGGA GGTGGAAGCTCCTCATAGAGCCCGCCCTCCAGAGCCCCGGCCACCCAGCACGCCTGCACCTCCTGTCAGA GATAGCTGTTCCTCCCCTCCCTCACTCAACTATGGGAAGGCCAAGGAGAAAACCATGGATGACTTGAAGT CTGAAGAATTAGCCAGGGAGATTGTGGGAAAGGACAAGTCTTTGGCTGACATCCTGGACCCCAGTGTGAA GATCAAAACTACCATGGATCTGATGGAGGGAATTTTTCCCAAAGATGAGTACCTCCTAGAGGAAGCTCAG CAGCGGAGAAAGCTGCTCCCCAAAGTCCCCTTACCCAGAGTCACAGAGGACAAGAAACAGGACCCAGGTA TGCCAGGGGTTGTGTCCTTGGCCACCAATTCTACCTATTACAGCACATCAGCCCCCAAAGCAGAGCTTCT TATCAAGATGAAGGACCTGCAGGAGCCTGAAGAGTATTCAGCAGGTGACTTGGATCATGACCTTTCTGTT AAGAAGCAAGAGCTCATTGACAGTATCAGCCGCAAGCTGCAGGTGCTCCGGGAAGCACGTGAGAGCCTGC TGGAAGACATCCAAGCCAACAATGCTCTTGGGGATGAGGTGGAAGCCATTGTGAAAGATGTCTGCAAGCC CAATGAGTTTGACAAGTTCCGGATGTTCATTGGAGACCTGGACAAAGTGGTGAACCTCCTGCTGTCACTG TCAGGACGCCTGGCCCGTGTGGAAAATGCCCTTAATAATTTAGATGACAATCCTTCTCCTGGAGATCGGC AGTCACTGTTGGAGAAACAGAGAGTCCTAACTCAGCAGCACGAGGATGCCAAGGAGCTTAAAGAGAACCT GGACCGCCGTGAGCGCATTGTGTTCGACATCCTGGCTACCTACCTCAGCGAGGAGAACCTGGCTGACTAT GAGCACTTCGTGAAGATGAAGTCAGCTCTCATCATTGAGCAGCGAGAGCTGGAAGATAAAATCCACCTGG GTGAAGAGCAGCTCAAGTGTTTGTTTGACAGCCTACAGCCTGAGAGAAGCAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290685
Insert Size	1386 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290685.1, NP_001277614.1
RefSeq Size	4826 bp
RefSeq ORF	1386 bp
Locus ID	110380
UniProt ID	A2ALU4
Gene Summary	May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution.[UniProtKB/Swiss-Prot Function]

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