Mid1 (NM_001290512) Mouse Untagged Clone

CAT#: MC227817

Mid1 (untagged) - Mouse midline 1 (Mid1), transcript variant 6



  "NM_001290512" in other vectors (1)

CNY 4,470.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 61B3-R; DXHXS1141; Fxy; Trim18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227817 representing NM_001290512
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCTTGCACAAGGATGCATTTTCAAAGCTTGACACTGAGCAAAACTTGGAGAGTAATCTCACCAATC
TTATTAAGAGAAACACAGAACTGGAGACTCTTTTGGCTAAACTCATCCAAACTTGTCAACATGTTGAAGT
CAATGCATCCCGTCAAGAAGCCAAACTGACAGAAGAATGTGATCTTCTCATTGAAATCATTCAGCAACGA
AGACAAATTATTGGAACAAAGATTAAAGAAGGCAAGGTGATCAGGCTCCGCAAGTTAGCTCAGCAGATTG
CAAACTGTAAACAGTGCCTTGAGAGGTCTGCATCGCTCATCTCGCAAGCGGAGCACTCGCTGAAGGAAAA
TGACCACGCCCGTTTTCTACAGACAGCAAAGAATATCACTGAGAGAGTCTCCATGGCAACTGCATCCTCC
CAGGTCCTAATTCCCGAAATCAACCTCAATGACACGTTTGACACTTTTGCCTTGGATTTTTCCCGGGAGA
AGAAACTGCTAGAATGTCTGGATTACCTAACAGCTCCCAACCCTCCCGCGATTAGAGAAGAGCTCTGCAC
CGCTTCCTACGACACCATCACCGTCCACTGGACCTCAGAGGACGAGTTCAGCGTGGTCTCCTACGAGCTC
CAGTACACCATATTCACCGGACAAGCCAATGTTGTCAGTCTGTGTAACTCGGCGGACAGCTGGATGATCG
TGCCCAACATCAAGCAGAACCACTACACCGTGCACGGCCTGCAAAGTGGCACCAAGTATATCTTCACGGT
GAAGGCCATCAACCAGGCGGGCAGCCGTAGCAGCGAGCCCGGAAAGCTGAAGACCAACAGTCAGCCGTTT
AGACTGGATCCCAAATCGGCTCATCGCAAGCTGAAGGTGTCCCACGACAACCTGACTGTCGAGCGCGACG
AGTCGTCCTCTAAGAAGAGTCACGCGCCGGAGCGCTTCGCTGGTCAGGGGAGCTACGGAGTGGCTGGCAA
CGTGTTCATCGACAGCGGCCGTCACTACTGGGAAGTGGTCACCAGCGGAAGCACATGGTACGCCATCGGC
CTGGCGTACAGATCGGCGCCGAAACACGAGTGGATCGGGAAGAACGCGGCGTCCTGGGCCCTCTGCCGCT
GCCACAACCACTGGGCGGTGCGACACGACGGCAAGGAGACCCCCATCGCGCCGGCCCCTCACCTCAGGCG
CGTCGGCGTCCTGCTGGACTACGACAACGGCTCCATCGCCTTCTACGACGCTCTGAGCTCCGTCCACCTC
CACACCTTCCACGCGGCGCTCGCGCAGCCCGTGTGCCCCACCTTCACCGTGTGGAACAAGTGTCTGACCA
TCGTCACGGGTCTGCCCATCCCGGACCATCTGGACTGTACGGAGCAGCGACCTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001290512
Insert Size 1386 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001290512.1, NP_001277441.1
RefSeq Size 3158 bp
RefSeq ORF 1386 bp
Locus ID 17318
Gene Summary Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses an alternate in-frame splice site, compared to variant 1. It initiates translation at an alternate start codon. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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