Tgfb1i1 (NM_001289550) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	ARA55; hic-5; Hic5; TSC-5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227816 representing NM_001289550 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACCTGGATGCCCTGCTCTCTGACCTGGAGACCACCACTTCACACATGTCACGGTTAGGGGCTC CAAAAGAGCGCCCACCAGAGACACTCACACCTCCCCCACCCTATGGCCACCAGCCACAGACAGGGTCTGG AGAATCTTCAGGAACCACTGGGGACAAGGATCATCTATACAGCACAGTATGCAAGCCTCGGTCCCCAAAG CCTGTGGCCCCTGTGGCTCCTCCATTCTCCTCTTCCAGTGGTGTGTTGGGCAATGGCCTCTGTGAGCTAG ACCGTTTGCTTCAGGAACTTAATGCCACCCAGTTCAACATTACAGATGAAATCATGTCTCAGTTCCCATC TAGTAAAATGGCTGAAGGGGAAGAGAAGGAGGACCAATCTGAAGACAAGAGCTCACCCACTGTCCCTCCC AGCCCATTCCCTGCCCCCTCAAAGCCTTCAGCCACCTCCGCCACTCAGGAACTGGATAGACTGATGGCCT CGCTCTCTGACTTCCGTGTTCAGAACCATCTTCCAGCCTCAGGGCCACCTCAGCCTCCAGCAGCGAGCCC CACCCGTGAAGGATGCCCATCTCCACCAGGACAGACTAGCAAAGGCAGTCTGGACACCATGCTGGGCCTG CTGCAGTCTGACCTCAGCCGTCGTGGTGTCCCCACACAGGCCAAAGGCCTCTGTGGCTCCTGCAATAAAC CTATAGCTGGGCAAGTGGTTACAGCCCTGGGCAGAGCCTGGCACCCGGAGCACTTCCTTTGCAGCGGTTG TTCCACAACCCTGGGAGGCAGCAGCTTCTTCGAGAAGGATGGGGCTCCCTTTTGCCCCGAGTGCTACTTT GAGCGCTTCTCCCCACGATGTGGCTTCTGTAACCAACCCATCCGACACAAAATGGTTACCGCCTTGGGCA CCCACTGGCACCCAGAACATTTCTGCTGCGTCAGCTGCGGAGAGCCTTTTGGAGAAGAGGGTTTCCACGA GCGGGAGGGTCGTCCGTACTGCCGGCGGGACTTCCTGCAGCTGTTCGCCCCACGCTGCCAGGGCTGCCAA GGCCCTATTTTGGATAACTACATCTCGGCACTCAGCGCGCTCTGGCACCCAGACTGCTTCGTGTGCAGGG AATGCCTTGCGCCCTTCTCCGGAGGCAGCTTTTTTGAGCACGAGGGTCGCCCTTTGTGTGAAAACCATTT CCATGCTCAGCGTGGTTCGCTGTGTGCCACGTGTGGTCTCCCAGTGACCGGCCGCTGTGTGTCCGCTCTG GGCCGACGCTTCCATCCAGACCACTTCACCTGCACATTCTGCCTGCGCCCACTCACCAAAGGCTCCTTCC AGGAGCGCGCCAGCAAGCCTTACTGCCAGCCTTGCTTCCTGAAGCTCTTCGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289550
Insert Size	1386 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289550.1, NP_001276479.1
RefSeq Size	3904 bp
RefSeq ORF	1386 bp
Locus ID	21804
UniProt ID	Q62219
Gene Summary	Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). CCDS Note: The coding region has been updated to represent an alternative 5' splicing pattern, resulting in an extended protein N-terminus that is more supported by the available transcript and conservation data.

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