Def8 (NM_001253784) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI449518; D8Ertd713e; DEF-8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227810 representing NM_001253784 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTCCTCATGCAGGGTGAAAGGGTGGGATGCTATGGAATATGATGAGAAGCTGGTTCGGTTCCGGC AGGCCCACCTCAACCCCTTCAACAAGCAGCTTGGTCCGAGGCATCATGAACAGGAACCCAGTGAGAAGGT CACTTCTGAAGACACTTTGCCTGAGCTGCCCGCTGGGGAGCCTGAATTCCACTACTCGGAGCGCATGATG GATCTCGGCCTGTCTGAGGACCACTTTTCCCGCCCTGTGGGTCTCTTCCTGGCCTCTGATGTCCAGCAGC TGCGGCAGGCCATCGAAGAATGCAAACAGGTGATCCTGGAGCTGCCCGAGCAGTCAGAGAAGCAGAAGGA CGCTGTGGTGCGGCTGATCCACCTCCGGCTGAAGCTCCAGGAGCTGAAGGACCCCAATGAGGAGGAACCC AACATCCGGGTTCTCCTGGAACATCGCTTCTACAAGGAGAAGAGCAAGAGCGTTAAACAGACCTGCGATA AGTGCAACACCATCATCTGGGGGCTCATCCAGACCTGGTACACCTGCACAGGGTGTTGTTACCGCTGTCA CAGCAAGTGCCTGAACCTCATCTCCAAACCATGTGTCAGCTCCAAGGTCAGTCACCAGGCTGAGTATGAG CTGAACATCTGCCCTGAGACCGGGCTGGACAGCCAGGACTACCGCTGTGCGGAGTGCCGTGCTCCCATCT CTCTGAGAGGTGTGCCTAGTGAGGCCCGGCAGTGTGACTACACGGGCCAATACTACTGCAGCCACTGCCA CTGGAACGACCTGGCTGTCATCCCCGCGAGAGTGGTGCACAACTGGGACTTTGAGCCACGCAAGGTGTCC CGTTGCAGCATGCGCTACCTGGCACTGATGGTGTCTCGGCCAGTGCTCCGGCTCCGGGAGATTAACCCTT TGCTGTTTAACTACGTGGAAGAGCTGGTGGAGATCCGGAAACTGCGCCAGGACATCCTGCTCATGAAGCC ATACTTCATCACCTGCAAGGAGGCCATGGAGGCGCGACTACTGCTGCAGCTCCAAGACAGACAGCATTTT GTGGAGAACGATGAGATGTACTCTATCCAGGACCTCTTGGAAGTGCACATGGGCCGCCTCAGCTGCTCGC TCACTGAGATCCACACGCTCTTCGCCAAGCACATCAAGTTGGACTGTGAGCGGTGCCAAGCCAAGGGGTT CGTCTGTGAACTCTGCAAAGAAGGGGACGTGCTCTTCCCGTTCGACAGCCACACGTCTGTGTGCAATGAC TGTTCGGCTGTCTTCCACAGGGACTGTTACTACGACAACTCGACCACGTGCCCCAAGTGTGCCCGGCTCA CATTGAGGAAGCAGTCACTATTCCAGGAACCTGGCCTAGACATGGATGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253784
Insert Size	1383 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253784.1, NP_001240713.1
RefSeq Size	3873 bp
RefSeq ORF	1383 bp
Locus ID	23854
UniProt ID	Q99J78
Gene Summary	Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (PubMed:27777970). Involved in bone resorption (PubMed:27777970).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate 5' exon structure, and thus differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at an alternate AUG and result in an isoform (2) with a shorter and distinct N-terminus, compared to isoform 1.

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