2010315B03Rik (NM_001243117) Mouse Untagged Clone

CAT#: MC227800

2010315B03Rik (untagged) - Mouse RIKEN cDNA 2010315B03 gene (2010315B03Rik), transcript variant 1



  "NM_001243117" in other vectors (1)

CNY 4,370.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms BC029716
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227800 representing NM_001243117
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACCTGGAAGACAGTCCTGCAACTTCTTTCAGGCTGGGCCCTGGCTGGTCAGTGCAAAGGAGAAGAC
ACCAGAGAAATGCAATGACCTATGCTGATGTGCATGTGAATTTCACTTGGGAAGAGTGGGCTTTGCTGAA
TCCTTCCCAGAAGAGTCTCTACAAAGAGGTGATGCTGGAGACCTACGAGAACCTCACTGCTATAGGCTAC
AATTGGGAAGACCATTATATTGAAGAACAATTTCAAAGTTCTAGAAGAGATGGAAGGCATAAAAGAGGTC
ACACTGGAGAGAAACCCTATGAATGTAATCATTGTGGTACAGCCTTTGCAAGGCATGGTCATCTGCAAAG
GCATAAAGGAACGCATACTGGAGAGAAACTTTATGAATGTAATCGATGTGGTAAAGTCTTTGCATGGAAC
TGTCATCTCCGAATCCATAAAAGAACACATAGTGGAGAGAAACCTTACGAATGTGATCAATGCGGTACAG
CCTTTGCAAGTCATTGTCGTCTTCAAAGGCATAAAAGAACACACACTGGAGAGAAACCTTATGAATGTAA
TCAATGTGGTAAAGCCTTTACACAACACAGTACTCTACAAGTTCATAAAAGAACACATACTGGAGAGAAA
CCCTATGAATGTAGCCAATGTGGTAAAGCCTATTCACGTCACAGTCATCTCCAAAGACATAAAAGAACAC
ATACTGGAGAGAAACCTTATGTATGTAATCAATGTGGTAAAGCCTTTGCTTATCAAAATAGTCTCCAGTA
TCATAAAAGAACACATACTGGAGAGAAATCTTATGAATGTAAGCAATGTGGTAAATTCTTTCCATGCCGT
AAGCATCTTCAAATACATAAAAGAACACATGCTGGACAGAAAGGCTATGAATGTAACAAATGTGGTAAAG
CCTTTGCTTTTCATAGTCATTTCCAAAGACATAAAAGACATATTGGAGAGAAACCTTATGAATGTAATCA
CTGTGATAAAGCCTTTGCAAGTCACAATAATCTTCAAAAACATATAAGAAGGCATACTGGAGAAAAACCT
TATAAATGTAATCTGTGTGACAAAGCCTATGCACATCACAGTCATCTCCAAACACATGAAAGAGTGCATA
CTGAAGAGAAACCCTACAAATGTAATCAATGTGATAAAGCCTTTTCACAACAAAGTTCTCTACAAGTGCA
TAAAAGAATACATACTGGAGAGAAACCCTATGAATGTAACCAATGTGGTAAAGCCTTTACATGTCACAGA
TATCTCCAAAGACATAAAAGAACACATACTGGAGAGAAACCCTATGAATGTAACCAATTTGGTAAAATAT
TTGCACAACATAGTACTGTCCACAGCATAAAAAAAGGAAATATTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001243117
Insert Size 1377 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001243117.1, NP_001230046.1
RefSeq Size 2700 bp
RefSeq ORF 1377 bp
Locus ID 630836
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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