Slc29a1 (NM_001199116) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1200014D21Rik; AA407560; ENT1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227799 representing NM_001199116 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACCAGTCACCAGCCTCAGGACAGGTATAAGGCAGTATGGCTTATCTTCTTTGTGCTGGGCCTGG GGACACTGCTCCCCTGGAATTTTTTTATGACCGCAACCAAGTATTTCACAAACCGCCTGGACGTGTCCCA GAATGTGTCCTCGGACACTGATCAATCATGCGAAAGCACCAAGGCCTTGGCTGACCCCACAGTGGCCTTG CCAGCCCGGAGTTCTCTCAGTGCCATCTTCAACAATGTCATGACCCTGTGTGCCATGCTGCCCTTGCTGG TCTTCACCTGCCTCAACTCGTTTCTGCATCAGCGGATCTCTCAATCTGTTCGGATCTTGGGCAGCCTGCT GGCAATCCTGCTGGTATTCCTTGTCACTGCCGCCCTGGTGAAGGTGGAGATGGATGCTCTGATCTTCTTT GTCATCACCATGATCAAGATTGTGCTCATCAATTCATTTGGTGCCATTTTGCAAGCCAGCCTTTTTGGTC TGGCAGGTGTCCTGCCAGCCAACTACACAGCCCCCATCATGAGTGGCCAGGGCCTGGCTGGCTTCTTCAC CTCTGTCGCCATGATCTGTGCCATTGCCAGTGGTTCTGAGCTGTCAGAAAGCGCCTTTGGCTACTTCATC ACAGCCTGTGCAGTTGTCATTTTGGCCATCCTGTGCTACCTGGCTCTGCCTCGGACGGAATTCTATCGCC ATTACCTGCAGCTCAACCTTGCGGGGCCTGCAGAGCAGGAGACCAAGTTGGATCTCATAAGAGAGGAGCC AAAAGGAAGAAGAGAGGAATCTGGGGTGCCAGGCCCCAACTCTCCACCCACCAACAGAAACCAGTCTATC AAAGCCATACTTAAGAGTATCTGTGTCCCGGCTCTGTCTGTCTGCTTCATCTTCACGGTTACCATTGGGT TGTTCCCTGCTGTGACTGCTGAGGTGGAATCCAGCATCGCAGGCACAAGTCCCTGGAAAAGCTACTTCAT TCCCGTGGCCTGTTTCTTGAATTTCAATGTCTTTGACTGGCTAGGCCGGAGCCTCACTGCTGTCTGCATG TGGCCTGGCCAGGATAGCCGCTGGCTGCCGGTTTTGGTCGCCTCGAGGATTGTGTTTATTCCCCTGCTGA TGCTCTGCAACGTGAAGGCTCGCCACTGCGGCGCGCAGCGGCACCACTTCGTCTTTAAGCATGACGCCTG GTTCATCGCCTTCATGGCTGCCTTTGCCTTCTCCAATGGCTACCTCGCCAGCCTCTGCATGTGCTTCGGG CCCAAGAAAGTCAAACCAGCTGAGGCGGAGACAGCAGGAAACATCATGTCCTTCTTTCTGTGTCTGGGCC TGGCTCTGGGAGCTGTGTTGTCCTTCTTGTTAAGGGCACTTGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199116
Insert Size	1377 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199116.1, NP_001186045.1
RefSeq Size	2006 bp
RefSeq ORF	1377 bp
Locus ID	63959
UniProt ID	Q9JIM1
Gene Summary	Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in the 5' UTR and uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes an isoform (2) that is two amino acids shorter than isoform 1. Variants 2, 4, and 5 encode the same protein.

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