Ebf1 (NM_001290711) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Ebf; O/E-1; OE-1; Olf-1; Olf1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227797 representing NM_001290711 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAAAACAAGCCATAGTGTATGAAGGCCAAGACAAGAACCCTGAAATGTGCCGAGTATTGCTCACAC ACGAGATCATGTGCAGCCGCTGTTGTGACAAGAAAAGCTGTGGCAACCGAAATGAGACTCCCTCAGATCC AGTGATAATTGACAGGTTCTTCCTGAAGTTTTTCCTTAAATGCAACCAAAATTGCCTAAAGAATGCAGGA AACCCACGTGACATGCGGAGATTCCAGGTCGTGGTGTCTACCACAGTCAACGTGGATGGCCATGTCCTGG CAGTCTCTGATAACATGTTTGTCCACAATAACTCCAAGCACGGGCGGAGGGCTCGGAGGCTTGACCCCTC GGAAGGTACGCCCTCTTATCTGGAACATGCTACTCCCTGTATCAAAGCCATCAGCCCGAGTGAAGGATGG ACGACGGGAGGCGCGACTGTGATCATCATAGGGGACAATTTCTTTGATGGGTTACAGGTCATATTCGGTA CCATGCTGGTCTGGAGTGAGTTGATAACTCCTCATGCCATCCGAGTTCAGACACCTCCTCGGCACATCCC TGGTGTGGTGGAAGTCACACTGTCGTACAAGTCCAAGCAGTTCTGCAAAGGGACACCAGGCAGATTCATC TACACAGCACTCAATGAACCCACCATCGACTACGGCTTCCAGAGGTTACAGAAGGTCATTCCTCGGCATC CTGGTGACCCAGAGCGCTTGCCAAAGGAAGTGATCCTTAAAAGAGCTGCAGATCTGGTTGAAGCCCTGTA TGGGATGCCCCACAACAACCAGGAGATTATCCTGAAGAGAGCTGCCGACATTGCAGAGGCTCTGTACAGT GTCCCTCGGAACCACAACCAGCTCCCAGCCCTTGCTAACACTTCGGTCCATGCAGGGATGATGGGTGTGA ACTCCTTCAGTGGACAACTGGCTGTGAATGTCTCGGAGGCATCACAAGCCACCAATCAAGGTTTCACCCG CAACTCAAGCAGCGTGTCACCACATGGCTATGTGCCGAGCACCACCCCACAGCAAACCAACTATAACTCA GTCACCACAAGCATGAATGGCTACGGCTCAGCCGCCATGTCCAATTTGGGCGGCTCCCCAACCTTCCTCA ATGGCTCAGCTGCCAACTCACCCTATGCCATTGTGCCATCCAGCCCCACCATGGCCTCATCTACAAGCCT CCCCTCCAACTGCAGTAGCTCCTCTGGCATCTTCTCCTTCTCACCAGCCAACATGGTCTCAGCAGTGAAA CAGAAGAGTGCTTTCGCACCAGTTGTCAGACCCCAGACGTCCCCTCCTCCCACCTGCACCAGCACCAACG GGAACAGCCTGCAAGCGATATCTGGCATGATTGTCCCTCCCATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290711
Insert Size	1377 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290711.1, NP_001277640.1
RefSeq Size	5322 bp
RefSeq ORF	1377 bp
Locus ID	13591
Gene Summary	Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in its 5' UTR, uses a downstream start codon, and uses an alternate splice site in the central coding region, compared to variant 1. The encoded isoform (4) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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