Erg (NM_001302154) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D030036I24Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227789 representing NM_001302154 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCACTATTAAGGAGGCCTTGTCAGTTGTGAGCGAGGACCAGTCACTATTTGAGTGTGCCTACG GAACGCCACACCTGGCTAAGACAGAGATGACCGCATCCTCTTCCAGTGACTATGGCCAGACATCCAAGAT GAGTCCCAGAGTCCCTCAGCAGGACTGGCTGTCTCAAGCCCCAGCCAGGGTCACCATCAAGATGGAGTGC AACCCTAGTCAGGTGAATGGTTCCAGGAACTCACCTGATGAGTGCAGTGTGAACAAAGGTGGGAAGATGG TGGGCAGCCCGGATACTGTGGGGATGAGCTACGGCAGCTACATGGAGGAGAAGCATGTGCCGCCTCCCAA TATGACCACAAATGAGCGCAGAGTGATCGTCCCTGCAGATCCTACTCTGTGGAGCACAGACCATGTCCGA CAGTGGCTGGAGTGGGCGGTGAAAGAATATGGCCTCCTCGATGTGGACGTCTTACTATTTCAGAATATCG ATGGGAAGGAGCTGTGCAAGATGACAAAGGATGACTTCCAGCGGCTCACGCCGAGCTACAATGCCGACAT TCTTCTCTCACATCTCCACTACCTCAGAGAGACTCCCCTTCCACATCTGACTTCCGATGACGTTGATAAG GCTTTACAAAACTCTCCACGGTTAATGCATGCCAGAAACACAGGGGGTGCAGCTTTTATTTTCCCAAATA CTTCAGTATATCCCGAAGCTACGCAAAGAATTACAACTAGGCCAGCTGCTCAGCCATCTCCCTCTGCAGT GCCCAAAACTGAAGACCAGCGTCCTCAGTTAGATCCTTACCAGATCCTGGGACCGACCAGTAGCCGCCTT GCTAATCCAGGTAGTGGCCAGATCCAGCTGTGGCAGTTCCTGCTCGAACTCCTGTCAGACAGCTCCAACT CCAACTGCATCACCTGGGAAGGCACCAACGGGGAGTTCAAGATGACAGACCCGGACGAGGTGGCTCGGCG CTGGGGGGAGAGGAAGAGCAAGCCCAACATGAACTATGACAAGCTCAGCCGCGCCCTCCGCTACTACTAC GACAAAAACATCATGACCAAGGTGCACGGGAAGCGCTACGCCTACAAGTTTGACTTCCACGGGATTGCCC AGGCCCTGCAGCCCCACCCTCCTGAGTCGTCCCTGTACAAGTACCCCTCCGACCTGCCATACATGGGCTC CTATCACGCCCACCCCCAGAAGATGAACTTTGTGTCTCCCCACCCTCCCGCTCTCCCAGTCACATCTTCC AGTTTCTTTGCTTCCCCGAACCCATACTGGAATTCACCGACTGGGGGCATCTACCCGAACACTAGGCTCC CAGCCAGCCATATGCCCTCTCACCTGGGCACCTACTACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302154
Insert Size	1371 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302154.1, NP_001289083.1
RefSeq Size	3121 bp
RefSeq ORF	1371 bp
Locus ID	13876
UniProt ID	P81270
Gene Summary	Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses a downstream alternate 5'-terminal exon which reflects use of an alternate promoter supported by epigenetic data, and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (4) is shorter and has a distinct N-terminus compared to isoform 1.

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