Vrk2 (NM_001252447) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2810003O05Rik; AI447698
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227777 representing NM_001252447 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCAAGAAGAAAAGAGAAATATAAGCTTCCGGTTCCACTCCCAGAAGGCAAAATTCTGGATGATA TGGAAGGAAACCGGTGGGCACTGGGCAAGATGATCGGCTCTGGAGGGTTTGGACTGATATACTTAGCTTT CCCCACAAATAAACCAAACAAAGATGCAAGACATGTCATAAAGCTGGAGTATCAAGAAAATGGCCCATTA TTTTCAGAGCTTAAATTTTATCAGAGAGCTGCAAAAAGAGAATGTATCCAAAAGTGGATACAACAGAGGA AACTTGATTATTTAGGAATTCCTGTATTTTATGGATTTGGTCTGACTGATTTCAAAGGAAGAAGTTACAG ATTCATGGTAATGGAAAGACTTGGAATAGATTTACAGAAACTCCTGGACCAGAATGGTGGTTTTAAAAAA TTAACCGTCCTACAACTTGGCATCAGGATGCTGGATGTACTGGAATATATACATGAAAATGAGTATGTTC ATGGTGATATAAAAGCCGCAAATCTACTGTTGGATTTTACAAATCCAGACCGGGTTTATCTTGCAGACTA TGGACTTTCCTACAGATATTGTCCCAATGGGAACCACAAACAGTATCAGGAAGATCCCAGAAAGGGCCAT AATGGGACAATAGAGTTTACAAGTTTGGATGCACACAAAGGAGTGGCCCCGTCCAGGAGGAGTGATGTTG AAATCCTTGGTTACTGCATGCTGCACTGGCTCTTCGGGAAGCTTCCTTGGGAAGCAAAGCTGGACGACCC TGTGGCTGTCCAGACTGCTAAAACCAACCTGCTGGATGAACTCCCAGAGTCGGTGCTTAAGTGGGCTCCT TCTGGAAGCAGTTGCAGTGAACTTGTCAAGTACTTGATGTATGTTCATAATTTAGCTTATGATGACAAGC CAGACTATCAGAAGCTCAAGAAAATTTTGAATCCAGATGGAGTACCTTTAGGGCCACTGGAATTTTCCAC TAAAGTACAGAGTGTCCATGTGCGGACTCCAGCCCAACAAAAAGAAAACTCAAGGACAAGGAAAATACAT GAATATTCTGATATTTTCAGTGAGATGCAGAGTCTTCAACAAACACCCAGCTATATGAGTTTCCAAGGTT CATATTGCAAGCCCTATCTGGACTGCACTAGAAGAGATCCCATCAGGAAGCCAAGATCCCTGCCTCGGTA CAGACACACGCCCACGGGTAATCTGGGAGTCACAGACTTGGAAAGTTCTCCAAGGTTTTGGCCTGCAATT TTTCAGTTGACTCTGAGTGAAGAGACAAAGGCAGATGTGTATTACTATGGGATCACTATATTCTGTCTGC TGATATTTGTATTTCTTGCTTTGTATTTTCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252447
Insert Size	1365 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252447.1, NP_001239376.1
RefSeq Size	1745 bp
RefSeq ORF	1365 bp
Locus ID	69922
UniProt ID	Q8BN21
Gene Summary	Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates histone H3. Phosphorylates 'Thr-18' of p53/TP53, and thereby increases its stability and activity. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. May also phosphorylate MAPK8IP1. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment in the C-terminal region, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.