Apbb1 (NM_001253887) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Fe65; Rir
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227765 representing NM_001253887 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGTACAGGACACCTCAGGGACCTACTACTGGCACATCCCAACAGGGACCACCCAGTGGGAACCCC CAGGCCGGGCCTCCCCCTCACAGGGGAGCAGCCCCCAAGAAGAGTCCCAGCTCACCTGGACTGGCTTTGC TCACCAAGAAGGCTTTGAGGAAGGAGAGTTTTGGAAGGATGAACCCAGTGAGGAGGCCCCAATGGAGTTG GGACTGAAGGACCCCGAGGAGGCGACATTGTCCTTCCCAGCTCAGAGCCTCAGCCCAGAACCAGTTCCCC AGGAGGAAGAGAAGCTGTCCCAACGGAATGCCAACCCAGGGATCAAGTGTTTCGCTGTGCGCTCCCTAGG CTGGGTAGAGATGACCGAGGAGGAGCTGGCCCCAGGACGCAGCAGTGTGGCAGTCAACAATTGTATCCGC CAGCTCTCCTACCACAAAAACAATCTACATGATCCGATGGCTGGGGGCTGGGGAGAGGGAAAGGATCTGC TGCTCCAGCTGGAGGACGAGACTCTAAAGTTGGTGGAGCCACAGAACCAGACGCTGCTGCATGCACAGCC CATCGTCAGCATTCGTGTGTGGGGCGTTGGGCGGGACAGTGGAAGAGAGAGGGACTTTGCCTACGTAGCT CGAGATAAGCTGACCCAGATGCTCAAGTGCCACGTGTTTCGCTGTGAGGCACCTGCCAAGAACATCGCCA CCAGCCTGCATGAGATCTGCTCCAAGATCATGTCTGAACGGCGCAATGCTCGCTGCTTGGTCAATGGACT CTCCCTAGACCACTCTAAACTCGTGGATGTCCCTTTCCAAGTGGAATTCCCAGCACCAAAGAATGAGCTG GTGCAGAAGTTCCAAGTCTATTACCTGGGAAATGTGCCAGTTGCTAAACCTGTTGGGGTAGACGTGATTA ATGGGGCCCTGGAGTCAGTCCTGTCTTCCAGTAGCCGTGAGCAGTGGACTCCAAGTCACGTCAGCGTGGC CCCTGCCACCCTCACCATCTTGCACCAGCAGACAGAAGCGGTGCTGGGGGAGTGCCGGGTGCGGTTTCTC TCCTTCCTGGCTGTGGGCAGAGATGTGCACACATTCGCGTTCATCATGGCTGCCGGCCCAGCCTCCTTCT GCTGTCACATGTTTTGGTGTGAGCCCAATGCTGCCAGTCTCTCAGAGGCTGTGCAGGCTGCATGCATGCT CCGCTACCAGAAGTGTCTGGATGCTCGCTCCCAGACCTCCACCTCCTGCCTCCCAGCACCCCCTGCGGAG TCAGTTGCAAGACGTGTAGGGTGGACAGTCCGCAGGGGTGTTCAGTCGCTGTGGGGTTCCCTCAAGCCCA AACGTCTGGGATCCCAGACCCCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253887
Insert Size	1356 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253887.1, NP_001240816.1
RefSeq Size	1922 bp
RefSeq ORF	1356 bp
Locus ID	11785
Gene Summary	Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and has multiple coding region differences compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (3) with a shorter N-terminus compared to isoform 1.

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