Pacsin1 (NM_001286744) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A830061D09Rik; H74; mKIAA1379; syndapin
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227723 representing NM_001286744 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGCTCCTACGATGAGGCCTCAGAGGAGATCACAGATAGCTTCTGGGAGGTGGGGAACTACAAGC GGACGGTGAAGCGCATCGACGATGGGCACCGCCTGTGCAACGACCTCATGAGCTGCGTGCAGGAGCGCGC CAAGATCGAGAAGGCATACGCGCAGCAGCTCACCGACTGGGCCAAGCGCTGGCGCCAGCTCATCGAGAAA GGTCCTCAGTATGGCAGCCTGGAGCGGGCGTGGGGCGCCATGATGACAGAAGCAGATAAGGTCAGCGAGC TGCACCAGGAGGTGAAGAACAGCCTGCTGAATGAGGACCTGGAGAAAGTCAAGAACTGGCAGAAGGATGC CTATCACAAGCAGATCATGGGTGGCTTCAAGGAGACGAAAGAGGCCGAGGATGGCTTCCGAAAGGCCCAG AAGCCCTGGGCTAAAAAGATGAAGGAGCTAGAGGCGGCCAAGAAGGCCTATCACTTGGCTTGTAAGGAGG AAAGGCTGGCCATGACCCGGGAGATGAACAGTAAGACAGAGCAGTCGGTCACCCCTGAACAGCAGAAGAA ACTTGTGGACAAAGTGGACAAATGCAGACAGGATGTGCAAAAGACTCAGGAGAAGTATGAGAAGGTGCTG GAAGATGTGGGCAAGACCACACCACAGTACATGGAGGGCATGGAGCAGGTGTTTGAGCAGTGCCAGCAGT TTGAGGAGAAGCGGCTGGTCTTCCTGAAGGAAGTCCTGCTGGATATCAAACGGCATCTCAACCTAGCGGA GAACAGCAGCTACATGCATGTCTACCGAGAACTGGAGCAGGCCATCCGGGGGGCCGATGCCCAGGAGGAC CTCAGGTGGTTCCGCAGCACCAGTGGCCCCGGGATGCCCATGAACTGGCCGCAGTTCGAGGAGTGGAACC CAGACCTCCCGCACACCACTGCCAAGAAGGAGAAACAGCCTAAGAAGGCAGAGGGGGCCACCCTGAGCAA TGCCACTGGGGCTGTAGAATCCACATCCCAGGCTGGGGACCGTGGCAGTGTTAGCAGCTATGACCGAGGC CAAACATATGCCACCGAGTGGTCAGACGATGAGAGCGGAAACCCCTTCGGGGGCAATGAGGCCAATGGTG GCGCCAACCCCTTCGAGGATGATGCCAAGGGAGTTCGTGTACGGGCACTCTATGACTACGACGGTCAGGA GCAGGATGAGCTCAGCTTCAAGGCCGGAGATGAGCTCACCAAGCTCGGAGAGGAAGACGAACAGGGTTGG TGCCGCGGGCGGCTGGACAGCGGACAGCTGGGCCTCTATCCTGCCAACTACGTTGAGGCTATATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286744
Insert Size	1326 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286744.1, NP_001273673.1
RefSeq Size	4156 bp
RefSeq ORF	1326 bp
Locus ID	23969
UniProt ID	Q61644
Gene Summary	Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) has an alternate splice site in the 5' UTR, compared to variant 3. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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