Erg (NM_001302183) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D030036I24Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227711 representing NM_001302183 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCAGACTGTACCTGACCCAGCAGCTCATATTAAGGAGGCCTTGTCAGTTGTGAGCGAGGACCAGT CACTATTTGAGTGTGCCTACGGAACGCCACACCTGGCTAAGACAGAGATGACCGCATCCTCTTCCAGTGA CTATGGCCAGACATCCAAGATGAGTCCCAGAGTCCCTCAGCAGGACTGGCTGTCTCAAGCCCCAGCCAGG GTCACCATCAAGATGGAGTGCAACCCTAGTCAGGTGAATGGTTCCAGGAACTCACCTGATGAGTGCAGTG TGAACAAAGGTGGGAAGATGGTGGGCAGCCCGGATACTGTGGGGATGAGCTACGGCAGCTACATGGAGGA GAAGCATGTGCCGCCTCCCAATATGACCACAAATGAGCGCAGAGTGATCGTCCCTGCAGATCCTACTCTG TGGAGCACAGACCATGTCCGACAGTGGCTGGAGTGGGCGGTGAAAGAATATGGCCTCCTCGATGTGGACG TCTTACTATTTCAGAATATCGATGGGAAGGAGCTGTGCAAGATGACAAAGGATGACTTCCAGCGGCTCAC GCCGAGCTACAATGCCGACATTCTTCTCTCACATCTCCACTACCTCAGAGAGACTCCCCTTCCACATCTG ACTTCCGATGACGTTGATAAGGCTTTACAAAACTCTCCACGGTTAATGCATGCCAGAAACACAGCTGCTC AGCCATCTCCCTCTGCAGTGCCCAAAACTGAAGACCAGCGTCCTCAGTTAGATCCTTACCAGATCCTGGG ACCGACCAGTAGCCGCCTTGCTAATCCAGGTAGTGGCCAGATCCAGCTGTGGCAGTTCCTGCTCGAACTC CTGTCAGACAGCTCCAACTCCAACTGCATCACCTGGGAAGGCACCAACGGGGAGTTCAAGATGACAGACC CGGACGAGGTGGCTCGGCGCTGGGGGGAGAGGAAGAGCAAGCCCAACATGAACTATGACAAGCTCAGCCG CGCCCTCCGCTACTACTACGACAAAAACATCATGACCAAGGTGCACGGGAAGCGCTACGCCTACAAGTTT GACTTCCACGGGATTGCCCAGGCCCTGCAGCCCCACCCTCCTGAGTCGTCCCTGTACAAGTACCCCTCCG ACCTGCCATACATGGGCTCCTATCACGCCCACCCCCAGAAGATGAACTTTGTGTCTCCCCACCCTCCCGC TCTCCCAGTCACATCTTCCAGTTTCTTTGCTTCCCCGAACCCATACTGGAATTCACCGACTGGGGGCATC TACCCGAACACTAGGCTCCCAGCCAGCCATATGCCCTCTCACCTGGGCACCTACTACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302183
Insert Size	1320 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302183.1, NP_001289112.1
RefSeq Size	3118 bp
RefSeq ORF	1320 bp
Locus ID	13876
UniProt ID	P81270
Gene Summary	Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (6) is shorter compared to isoform 1.

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