Hnrnpk (NM_001301345) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	hn; Hnrpk; KBB; KBBP; NOVA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227707 representing NM_001301345 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCGAACAGCCAGAAGAAACCTTCCCCAACACCGAAACCAATGGTGAATTTGGTAAACGCCCTG CAGAAGATATGGAAGAGGAGCAAGCCTTTAAAAGATCTAGAAATACTGATGAGATGGTTGAATTGCGCAT TTTGCTTCAGAGCAAGAATGCTGGAGCAGTGATTGGAAAAGGAGGCAAGAATATTAAGGCTCTCCGTACA GACTACAATGCCAGTGTTTCAGTCCCAGACAGCAGTGGCCCCGAGCGCATACTGAGTATCAGTGCTGATA TTGAGACGATTGGAGAAATTCTGAAGAAAATCATCCCTACCTTGGAAGAGTACCAACATTATAAAGGAAG TGACTTTGATTGCGAGTTGAGACTGTTGATTCATCAGAGTCTGGCAGGAGGAATAATTGGTGTTAAAGGT GCTAAAATCAAAGAACTTCGAGAAAACACTCAGACAACAATCAAGCTTTTCCAGGAGTGCTGCCCTCACT CTACTGACAGAGTTGTTCTTATTGGAGGAAAACCTGATAGGGTTGTAGAATGCATCAAGATCATCCTTGA CCTTATATCTGAGTCTCCCATCAAAGGACGTGCACAACCTTATGATCCCAACTTTTATGATGAGACCTAT GATTATGGTGGTTTTACAATGATGTTTGATGACCGCCGAGGACGACCTGTGGGATTCCCCATGAGGGGAA GAGGTGGTTTTGACAGAATGCCTCCTGGTCGGGGTGGGCGTCCCATGCCTCCTTCTAGAAGAGATTATGA TGATATGAGCCCTCGTCGAGGACCTCCACCACCACCACCTGGTCGAGGTGGCCGGGGTGGCAGCAGAGCC CGGAATCTGCCTCTTCCTCCTCCACCACCACCCAGAGGGGGAGATCTAATGGCTTATGACAGAAGAGGAA GGCCTGGAGACCGCTATGATGGCATGGTTGGGTTCAGTGCTGATGAAACTTGGGATTCTGCAATTGACAC ATGGAGCCCATCAGAATGGCAAATGGCTTATGAACCACAGGGTGGTTCTGGATATGACTATTCTTATGCA GGGGGCCGTGGCTCATATGGTGATCTTGGCGGACCTATTATCACTACACAAGTAACTATTCCCAAAGATT TGGCTGGATCTATTATTGGCAAAGGTGGTCAGCGGATTAAACAAATTCGTCATGAATCTGGAGCATCAAT CAAAATTGATGAACCTTTAGAAGGATCTGAAGATCGGATCATTACCATTACAGGAACACAGGACCAGATA CAGAACGCACAGTATTTGCTGCAGAACAGTGTGAAGCAGTATTCTGGAAAGTTTTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301345
Insert Size	1320 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301345.1, NP_001288274.1
RefSeq Size	2721 bp
RefSeq ORF	1320 bp
Locus ID	15387
UniProt ID	P61979
Gene Summary	The protein encoded by this gene is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex, and is a poly-cytosine binding protein (PCBP). It is found in multiple subcellular compartments including the nucleus, cytoplasm and mitochondria. This gene product is thought to interact with RNA, DNA, and protein, and is involved in multiple cellular processes, including transcription, chromatin remodeling, DNA damage response, signal transduction, mRNA splicing, export, and translation. Multiple transcript variants and protein isoforms exist, with some isoforms containing a unique C-terminus. There are four pseudogenes of this gene, found on chromosomes 2, 3, 7 and 13. [provided by RefSeq, Aug 2014] Transcript Variant: This variant (6) lacks an in-frame exon and uses an alternative splice site in the 3' terminal exon, which results in a frameshift, compared to variant 1. It encodes isoform 4 which is shorter and has a distinct C-terminus compared to isoform 1.

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