Klf10 (NM_001289471) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI115143; Egral; EGR[a]; Gdnfif; mGIF; Tieg; TIEG-1; Tieg1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227679 representing NM_001289471 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAACTTCGGCGCTTCTCTCCAGCAAGCTTCGGAGGGGAAAATGGAACTAATTTCTGAAAAGCCCA GAGAGGGGATGCATCCCTGGGACAAAGCTGAGCAGAGTGACTTTGAAGCGGTGGAAGCGCTCATGTCCAT GAGCTGCGACTGGAAGTCTCATTTCAAGAAATACCTTGAAAACAGGCCTGTCACACCAGTGTCTGATACC TCCGAGGATGACAGCTTGCTTCCAGGGACGCCTGACCTTCAGACAGTCCCAGCATTTTGTTTAACGCCAC CTTACAGCCCCTCTGACTTCGAACCCTCCCAAGGGTCAAATCTGACTGCATCAGCGCCATCTACTGGCCA CTTCAAATCTTTCTCCGATGCTGCCAAGCCTCCAGGCGCCACTCCTTTCAAAGAGGAGGAAAAGAATCCT TTAGCTGCCCCTCCTCTTCCTAAGGCTCAAGCCACCAGTGTCATCCGTCACACAGCTGATGCCCAACTGT GCAACCACCAGTCCTGCCCCGTGAAAGCAGCTAGCATCCTCAACTATCAGGACAATTCTTTCCGGAGAAG AACCCACGGAAATGTTGAGGCTACTCGAAAGAACATACCCTGTGCTGCAGTGTCACCAAACAGATCCAAG CCTGAGCCCAGCACAGTGTCCGATGGTGATGAGAAGGCGGGCGCTGCACTATATGACTTTGCTGTGCCTT CCTCAGAGACAGTAATTTGTAGGTCTCAGCCAGCTCCTTCGTCCCCAGTGCAGAAGTCAGTACTGGTGTC TTCACCTACAGTATCCACTGGGGGAGTGCCACCCCTGCCTGTCATCTGCCAGATGGTTCCCCTTCCTGCC AACAACTCTCTTGTTAGCACAGTTGTCCCCAGCACTCCTCCTAGCCAGCCACCAGCTGTCTGCTCACCTG TGTTGTTCATGGGCACTCAGGTGCCTGAGGGCACCGTCGTGTTTGTGGTACCCCAGCCCGTTGTGCAGAG CCCAAGGCCTCCAGTGGTGAGCCCCAGTGGCACCAGACTGTCTCCCATTGCCCCTGCTCCTGGATTCTCT CCTTCAGCAGCAAGGGTCACTCCTCAGATTGACTCGTCCAGAGTAAGAAGTCACATCTGTAGCCACCCAG GGTGTGGCAAGACTTACTTTAAAAGTTCCCATCTGAAGGCCCACGTGAGGACACACACAGGGGAAAAACC TTTCAGCTGCAGCTGGAAAGGCTGTGAAAGGAGGTTTGCTCGCTCCGATGAACTGTCCAGACACCGGCGG ACACACACAGGAGTCTCTGAGTCTTGCCCTCAGCATCTCGCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289471
Insert Size	1305 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289471.1, NP_001276400.1
RefSeq Size	2148 bp
RefSeq ORF	1305 bp
Locus ID	21847
Gene Summary	Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. May play a role in the cell cycle regulation (By similarity). Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR and 3' coding region compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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