Rcc1 (NM_001197082) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4931417M11Rik; AI326872; Chc1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227676 representing NM_001197082 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACCCAAGCGCATAGCTAAGAGAAGGTCACCCCCAGAAGATGCCATCCCCAAAAGCAAGAAGGTGA AAGACACTCGTAATCGCGGTCCTGCTACTCGCTCGTGCCAAGTCTCACACAGGTCCCACAACACAGAACC AGGCTTGGTGCTGACACTGGGCCAGGGCGACGTGGGCCAGCTGGGGCTGGGTGAAAGTGTGCTGGAGAGG AAGAAGCCGGCCTTGGTGCCCCTTCTGCAGGATGTTGTGCAGGCTGAGGCCGGGGGCATGCATACTGTGT GTCTGAGCCAAAGTGGCCAGGTCTACTCCTTTGGCTGCAATGACGAGGGTGCCCTGGGAAGGGACACTTC AGTCGAAGGCTCAGAAATGGTCCCTGGGAAAGTGGAACTGCAAGAGAAAGTGGTGCAAGTGTCAGCGGGG GACAGTCACACAGCAGCCCTCACCGAAGACGGCCGTGTCTTCCTTTGGGGCTCTTTCCGGGACAATAATG GTGTGATCGGGTTGTTGGAGCCCATGAAGAAGAGCATGGTACCTGTTCAAGTGCAGCTGGATGCGCCCGT GGTAAAGGTGGCCTCGGGGAACGACCACTTGGTGATGCTCACGAACGATGGCGACCTCTACACCTTGGGT TGTGGGGAGCAAGGTCAGCTGGGCCGTGTACCTGAATTATTTGCCAACCGAGGTGGCCGTCAGGGCCTTG GGCGACTCCTGGTCCCCAGATGTGTGCTGCTGAAATCCAGGGGGACCCGGGGCCGTGTGCGGTTCCAGGA TGCCTTCTGTGGCGCCTATTTCACTTTTGCCATCTCCCGAGAGGGCCACGTGTATGGCTTTGGCCTCTCC AACTATCACCAGCTCGGGACTCCAGGCACTGGATCTTGCTTCATTCCTCAAAACTTGACATCCTTCAAGA ATTCCACCAAGTCCTGGGTAGGCTTCTCCGGTGGCCAGCATCACACAGTCTGCATGGATTCGGAAGGGAA GGCGTACAGCCTGGGCCGGGCTGAGTACGGGCGCCTGGGCCTTGGGGAGGGTGCTGAGGAGAAGAGCATA CCCACCCTCATTTCCCGGCTGCCCGTGGTCTCCTCAGTGGCCTGTGGGGCTTCTGTGGGTTATGCTGTGT CCAAGGATGGTCGTGTTTTTGCCTGGGGCATGGGCACCAACTACCAGCTGGGCACCGGGCAGGATGAAGA TGCCTGGAGTCCTGTGGAAATGACTGGCAAACAGCTGGAGAACCGTGTGGTGTTAACTGTGTCCAGTGGG GGCCAGCACACGGTCTTACTAGTTAAGGACCAGGCACAGAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001197082
Insert Size	1305 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001197082.1, NP_001184011.1
RefSeq Size	2332 bp
RefSeq ORF	1305 bp
Locus ID	100088
Gene Summary	Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis. Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis. Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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